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| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAP |
| Uniprot No | Q13114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-568aa |
| 氨基酸序列 | MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP |
| 预测分子量 | 64.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAP(Latency-Associated Peptide)重组蛋白的3篇参考文献及摘要概括：
1. **文献名称**：*Recombinant Latency-Associated Peptide of TGF-β1 modulates fibrosis in experimental models*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统重组表达TGF-β1的LAP蛋白，验证其抑制纤维化的功能，发现LAP通过阻断TGF-β1活性减轻小鼠肝纤维化。
2. **文献名称**：*Expression and purification of functional LAP (TGF-β1) in a mammalian cell system*
**作者**：Chen L, et al.
**摘要**：报道利用哺乳动物HEK293细胞表达重组LAP蛋白，优化纯化工艺并验证其与TGF-β受体的结合能力，为疾病治疗提供高活性蛋白来源。
3. **文献名称**：*LAP-mediated immune tolerance in cancer therapy*
**作者**：Wang Y, et al.
**摘要**：探讨重组LAP蛋白在肿瘤微环境中调节免疫耐受的作用，实验表明LAP抑制T细胞过度活化，增强免疫检查点抑制剂疗效。
4. **文献名称**：*Structural analysis of recombinant LAP and its role in TGF-β activation*
**作者**：Johnson R, et al.
**摘要**：通过X射线晶体学解析重组LAP蛋白结构，揭示其与TGF-β1结合的分子机制，为靶向药物设计提供结构基础。
(注：以上文献为模拟示例，实际引用需查询具体数据库如PubMed。)
**Background of LAP Recombinant Protein**
LAP (Latency-Associated Peptide) recombinant protein is derived from the N-terminal region of the latent transforming growth factor-beta (TGF-β) precursor, primarily associated with TGF-β1. TGF-β1 is a multifunctional cytokine involved in cell proliferation, differentiation, apoptosis, and immune regulation. However, TGF-β1 is secreted in a latent complex, where LAP non-covalently binds to mature TGF-β, rendering it inactive. This latent form ensures controlled activation, preventing unchecked TGF-β signaling, which is implicated in fibrosis, cancer progression, and autoimmune disorders.
Recombinant LAP proteins are engineered using biotechnological methods, such as expression in mammalian or bacterial systems (e.g., *E. coli*), followed by purification. These proteins retain the ability to interact with TGF-β or its receptors, making them valuable tools for studying TGF-β activation mechanisms. For instance, LAP recombinant proteins are used to block TGF-β activity in experimental models, elucidating its role in diseases like tissue fibrosis or tumor immunosuppression.
Additionally, LAP has been explored in therapeutic contexts. By leveraging its binding properties, recombinant LAP can be conjugated to drug carriers or scaffolds to target TGF-β-rich microenvironments, such as injured tissues or tumors. Recent studies also highlight its potential in regenerative medicine, where modulating TGF-β signaling via LAP could promote tissue repair while minimizing fibrotic side effects.
Overall, LAP recombinant protein serves as a critical reagent for both basic research and translational applications, offering insights into TGF-β biology and pathways for developing targeted therapies. Its versatility underscores its importance in understanding and manipulating cellular responses in health and disease.
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