| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cell division control protein 45 homolog, PORC-PI-1, CDC45, CDC45L, CDC45L2 |
| Entrez GeneID | 8318 |
| WB Predicted band size | 65.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the Central region of human CDC45L. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDC45抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*CDC45 promotes cell cycle progression by regulating CDC25A stability in human cholangiocarcinoma*
**作者**：Li Y, et al.
**摘要**：该研究使用CDC45抗体通过Western blot和免疫组化分析了CDC45在胆管癌中的表达水平，发现其过表达通过稳定CDC25A蛋白促进细胞周期G1/S期转换，提示CDC45可能作为胆管癌治疗的潜在靶点。
2. **文献名称**：*A role for CDC45 in the dynamics of DNA replication during the Xenopus laevis embryonic cell cycle*
**作者**：Pacek M, Walter JC.
**摘要**：作者利用非洲爪蟾胚胎提取物模型，通过抗CDC45抗体的免疫共沉淀实验，揭示了CDC45在DNA复制起始复合体中的动态作用，证明其与MCM复合物及GINS的相互作用对复制叉的形成至关重要。
3. **文献名称**：*CDC45 depletion inhibits neuroblastoma cell proliferation by inducing cell cycle arrest and apoptosis*
**作者**：Zhang H, et al.
**摘要**：研究通过siRNA敲低CDC45并结合抗CDC45抗体的免疫荧光实验，发现CDC45缺失导致神经母细胞瘤细胞G2/M期阻滞及凋亡增加，提示其在肿瘤增殖中的关键调控作用。
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注：以上文献信息为示例，实际引用时需核对真实来源。若需精准文献，建议通过PubMed/Google Scholar检索关键词“CDC45 antibody”或联系领域专家获取。
The CDC45L antibody is a crucial tool for studying the CDC45 (Cell Division Cycle 45) protein, a key regulator of DNA replication and cell cycle progression. CDC45. encoded by the CDC45L gene, is an essential component of the eukaryotic replisome, working alongside the MCM complex and GINS proteins to form the CMG helicase, which unwinds DNA during replication. It plays a pivotal role in the initiation and elongation phases of DNA synthesis, ensuring proper replication fork progression and genome stability. Dysregulation of CDC45 is linked to replication stress, chromosomal abnormalities, and diseases like cancer.
CDC45L antibodies are widely used in molecular biology to detect and quantify CDC45 expression in various experimental models, including Western blotting, immunofluorescence, and immunohistochemistry. These antibodies help elucidate CDC45's spatiotemporal dynamics during the cell cycle, its interaction partners, and its role in replication checkpoint mechanisms. Researchers also employ CDC45L antibodies to investigate its overexpression or mutations in cancer cells, providing insights into oncogenic proliferation and potential therapeutic targets. Validated for specificity and sensitivity, high-quality CDC45L antibodies are critical for advancing studies on DNA replication fidelity, cell cycle control, and cancer biology.
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