| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGSF8 |
| Uniprot No | Q969P0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-579aa |
| 氨基酸序列 | ADLREVLVPEGPLYRVAGTAVSISCNVTGYEGPAQQNFEWFLYRPEAPDT ALGIVSTKDTQFSYAVFKSRVVAGEVQVQRLQGDAVVLKIARLQAQDAGI YECHTPSTDTRYLGSYSGKVELRVLPDVLQVSAAPPGPRGRQAPTSPPRM TVHEGQELALGCLARTSTQKHTHLAVSFGRSVPEAPVGRSTLQEVVGIRS DLAVEAGAPYAERLAAGELRLGKEGTDRYRMVVGGAQAGDAGTYHCTAAE WIQDPDGSWAQIAEKRAVLAHVDVQTLSSQLAVTVGPGERRIGPGEPLEL LCNVSGALPPAGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGVGSLGPGY EGRHIAMEKVASRTYRLRLEAARPGDAGTYRCLAKAYVRGSGTRLREAAS ARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLR LAASWWVERPEDGELSSVPAQLVGGVGQDGVAELGVRPGGGPVSVELVGP RSHRLRLHSLGPEDEGVYHCAPSAWVQHADYSWYQAGSARSGPVTVYPYM HALDTHHHHHH |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGSF8重组蛋白的3篇参考文献示例(注：文献为虚拟示例，实际研究中请核实真实来源)：
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1. **文献名称**：*Recombinant IGSF8 promotes cell-cell adhesion through homophilic interactions*
**作者**：Tanaka H, et al.
**摘要**：本研究成功表达并纯化了重组IGSF8蛋白，证实其通过同源相互作用介导细胞间粘附。实验表明，重组IGSF8可增强上皮细胞的紧密连接，并参与调控细胞迁移过程。
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2. **文献名称**：*Structural and functional characterization of IGSF8 recombinant protein in immune regulation*
**作者**：Liu X, Wang Y.
**摘要**：通过哺乳动物表达系统制备了重组IGSF8胞外段蛋白，解析其晶体结构，发现其免疫球蛋白结构域与T细胞表面受体CD226存在特异性结合，提示其在免疫突触形成中的潜在作用。
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3. **文献名称**：*IGSF8 recombinant protein suppresses tumor metastasis by modulating integrin signaling*
**作者**：Park S, et al.
**摘要**：研究利用重组IGSF8蛋白处理多种癌细胞系，发现其通过抑制整合素β1信号通路降低肿瘤细胞的侵袭能力，为靶向IGSF8的癌症治疗策略提供依据。
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**备注**：以上文献为示例性内容，实际研究中建议通过PubMed、Web of Science等数据库检索最新文献，或参考领域内权威期刊(如*Journal of Biological Chemistry*、*Cell Communication and Adhesion*等)。
IGSF8 (Immunoglobulin Superfamily Member 8), also known as PGRL or SynCAM3. is a transmembrane protein belonging to the immunoglobulin superfamily (IgSF). It is characterized by extracellular immunoglobulin (Ig)-like domains that mediate cell-cell adhesion and signaling. IGSF8 is broadly expressed in various tissues, including the immune system, brain, and epithelial cells, suggesting roles in both neurological and immunological processes. Functionally, it has been implicated in modulating immune responses, synaptic organization, and tumorigenesis. Studies highlight its interaction with proteins like CRTAM (Class I-restricted T cell-associated molecule) and its potential involvement in regulating T-cell activity, natural killer (NK) cell function, and dendritic cell maturation.
Recombinant IGSF8 protein is typically engineered to include specific functional domains, such as the extracellular Ig-like regions, to study its binding partners and signaling mechanisms. This protein is produced using expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and structural integrity. Purification often involves affinity tags (e.g., His-tag or Fc-fusion) for experimental flexibility. Researchers utilize recombinant IGSF8 to investigate its role in immune synapse formation, cancer cell adhesion, and metastasis, as well as its therapeutic potential in autoimmune diseases or cancer immunotherapy. Recent studies also explore its crosstalk with immune checkpoints like PD-1/PD-L1. positioning IGSF8 as a novel target for immune modulation. Its recombinant form serves as a critical tool for structural studies, antibody development, and functional assays in vitro and in vivo.
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