| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNTN2 |
| Uniprot No | Q02246 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-1012aa |
| 氨基酸序列 | SQTTFGPVFE DQPLSVLFPE ESTEEQVLLA CRARASPPAT YRWKMNGTEM KLEPGSRHQL VGGNLVIMNP TKAQDAGVYQ CLASNPVGTV VSREAILRFG FLQEFSKEER DPVKAHEGWG VMLPCNPPAH YPGLSYRWLL NEFPNFIPTD GRHFVSQTTG NLYIARTNAS DLGNYSCLAT SHMDFSTKSV FSKFAQLNLA AEDTRLFAPS IKARFPAETY ALVGQQVTLE CFAFGNPVPR IKWRKVDGSL SPQWTTAEPT LQIPSVSFED EGTYECEAEN SKGRDTVQGR IIVQAQPEWL KVISDTEADI GSNLRWGCAA AGKPRPTVRW LRNGEPLASQ NRVEVLAGDL RFSKLSLEDS GMYQCVAENK HGTIYASAEL AVQALAPDFR LNPVRRLIPA ARGGEILIPC QPRAAPKAVV LWSKGTEILV NSSRVTVTPD GTLIIRNISR SDEGKYTCFA ENFMGKANST GILSVRDATK ITLAPSSADI NLGDNLTLQC HASHDPTMDL TFTWTLDDFP IDFDKPGGHY RRTNVKETIG DLTILNAQLR HGGKYTCMAQ TVVDSASKEA TVLVRGPPGP PGGVVVRDIG DTTIQLSWSR GFDNHSPIAK YTLQARTPPA GKWKQVRTNP ANIEGNAETA QVLGLTPWMD YEFRVIASNI LGTGEPSGPS SKIRTREAAP SVAPSGLSGG GGAPGELIVN WTPMSREYQN GDGFGYLLSF RRQGSTHWQT ARVPGADAQY FVYSNESVRP YTPFEVKIRS YNRRGDGPES LTALVYSAEE EPRVAPTKVW AKGVSSSEMN VTWEPVQQDM NGILLGYEIR YWKAGDKEAA ADRVRTAGLD TSARVSGLHP NTKYHVTVRA YNRAGTGPAS PSANATTMKP PPRRPPGNIS WTFSSSSLSI KWDPVVPFRN ESAVTGYKML YQNDLHLTPT LHLTGKNWIE IPVPEDIGHA LVQIRTTGPG GDGIPAEVHI VRNGGTSMMV EN |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNTN2重组蛋白的3篇参考文献及其简要摘要：
---
1. **文献名称**：*CNTN2 promotes neural metastasis in lung adenocarcinoma via triggering epithelial-mesenchymal transition*
**作者**：Li Y, et al.
**摘要**：该研究通过重组CNTN2蛋白实验，发现其过表达可诱导肺癌细胞上皮-间质转化(EMT)，促进肿瘤神经侵袭和转移，揭示了CNTN2在肺癌转移中的分子机制。
---
2. **文献名称**：*Recombinant CNTN2 facilitates axon regeneration by activating the PI3K/Akt signaling pathway*
**作者**：Zhang H, et al.
**摘要**：研究利用重组CNTN2蛋白处理脊髓损伤模型，发现其通过激活PI3K/Akt通路促进轴突再生，为神经修复治疗提供了潜在靶点。
---
3. **文献名称**：*Structural characterization of human CNTN2 extracellular domain by X-ray crystallography*
**作者**：Wang Q, et al.
**摘要**：通过重组表达并纯化CNTN2胞外结构域，结合X射线晶体学解析其三维结构，揭示了其与配体相互作用的分子基础，为药物设计提供结构依据。
---
4. **文献名称**：*CNTN2 recombinant protein ameliorates cognitive deficits in Alzheimer's disease mice*
**作者**：Chen L, et al.
**摘要**：实验表明，脑内注射重组CNTN2蛋白可改善阿尔茨海默病模型小鼠的突触可塑性和认知功能，提示其在神经退行性疾病中的治疗潜力。
---
注：以上文献信息为示例性内容，实际引用需以具体论文数据为准。建议通过PubMed或Web of Science以“CNTN2 recombinant protein”为关键词检索最新研究。
Contactin-2 (CNTN2), also known as TAG-1 (transient axonal glycoprotein-1) or AXLIG, is a neural cell adhesion molecule belonging to the immunoglobulin (Ig) superfamily. Structurally, it consists of six Ig-like domains and four fibronectin type III repeats anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage. CNTN2 plays critical roles in nervous system development, including axon guidance, myelination, and synapse formation. It mediates homophilic or heterophilic interactions with partners like L1CAM or Contactin-associated protein 1 (Caspr1) to regulate neuronal network assembly and signal transduction.
In disease contexts, CNTN2 is implicated in neurodevelopmental disorders, autoimmune conditions, and cancers. Studies link its dysregulation to multiple sclerosis, schizophrenia, and glioma progression. Its expression in non-neuronal tissues, such as immune cells and tumor microenvironments, suggests broader functional roles in cell adhesion, migration, and immune modulation.
Recombinant CNTN2 protein is engineered using mammalian or insect cell systems to preserve post-translational modifications and ligand-binding capacity. It serves as a vital tool for investigating molecular mechanisms in neurobiology, screening therapeutic agents, or developing diagnostic biomarkers. Purification typically involves affinity chromatography with tags like Fc or His. Researchers utilize it in vitro (e.g., neurite outgrowth assays) and in vivo (disease modeling) to explore CNTN2's pathophysiological contributions. Recent interest focuses on its potential as a cancer immunotherapy target due to aberrant expression in metastatic cells.
×
