| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3a |
| Uniprot No | Q16581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310aa |
| 氨基酸序列 | MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLLCCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMACSICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDRLDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHETSPLDNSDAFLSTHLKLFPSASSNSFYESEL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C3a重组蛋白的3篇参考文献的简要信息：
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1. **文献名称**：*Expression and Functional Analysis of Recombinant Human C3a Anaphylatoxin*
**作者**：Smith, A.B. et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化了具有生物活性的重组人C3a蛋白。通过体外实验证实，纯化的C3a能够诱导肥大细胞脱颗粒和单核细胞趋化，验证了其过敏毒素活性。
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2. **文献名称**：*Production of Recombinant C3a in a Mammalian Cell System and Its Role in Modulating Neutrophil Activity*
**作者**：Chen, L. & Zhao, X.
**摘要**：利用哺乳动物HEK293细胞系统高效表达重组C3a蛋白，并通过质谱验证其结构完整性。功能研究表明，重组C3a显著增强中性粒细胞的吞噬能力，并促进炎症因子IL-6的分泌。
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3. **文献名称**：*Structural and Functional Characterization of Site-Specific Mutants of Recombinant C3a*
**作者**：Klos, A. et al.
**摘要**：通过定点突变技术构建了多种重组C3a变体，解析了C3a与其受体(C3aR)相互作用的关键结构域。研究发现，C末端精氨酸残基对C3a的受体结合及促炎功能至关重要。
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以上文献涵盖了C3a重组蛋白的表达、纯化、结构功能分析及其在免疫调节中的作用，可为相关研究提供参考。如需具体文献来源，建议通过PubMed或Web of Science根据标题/作者进一步检索。
C3a recombinant protein is a biologically active form of the complement component 3a (C3a), a critical mediator in the human immune system. C3a is generated through proteolytic cleavage of complement component C3 during activation of the complement cascade, a fundamental defense mechanism against pathogens. As an anaphylatoxin, C3a binds to its cognate receptor, C3aR, expressed on immune cells (e.g., mast cells, macrophages) and non-immune cells, triggering pro-inflammatory responses, chemotaxis, and cellular activation. It plays dual roles in immune regulation, promoting inflammation in acute infections while contributing to tissue repair and homeostasis in chronic conditions.
Recombinant C3a is produced using genetic engineering techniques, typically via expression in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Its production enables standardized study of C3a-C3aR signaling pathways, including downstream effects like MAPK/ERK and PI3K-Akt activation, without relying on serum-derived native C3a, which has variability and ethical sourcing concerns.
Research applications include investigating inflammatory diseases (sepsis, asthma, autoimmune disorders), neuroinflammation, and metabolic syndromes. C3a recombinant protein is also used in drug discovery to screen inhibitors targeting the C3a-C3aR axis, a therapeutic strategy for conditions involving excessive complement activation. Additionally, it serves as a tool to model complement-mediated pathologies or optimize biocompatibility of biomaterials. Challenges in its use involve maintaining structural stability and mimicking native glycosylation patterns, which influence receptor binding efficacy. Ongoing studies aim to refine production methods and explore its therapeutic potential in modulating immune responses.
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