| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | B2R; BK2; BK-2; BKR2; BRB2 |
| WB Predicted band size | 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human BDKRB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于Phospho-eNOS(S1177)抗体的参考文献摘要概览：
1. **"Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation"**
- 作者：Dimmeler S, et al.
- 摘要：研究揭示了Akt激酶通过磷酸化eNOS的S1177位点(人源为S1179)激活其酶活性，促进一氧化氮(NO)生成，对血管舒张和血管生成至关重要。实验中使用了Phospho-eNOS(S1177)抗体验证磷酸化修饰。
2. **"Regulation of endothelium-derived nitric oxide production by the protein kinase Akt"**
- 作者：Fulton D, et al.
- 摘要：阐明了Akt介导的eNOS S1177磷酸化在血流剪切应力或胰岛素刺激下的作用，Phospho-eNOS(S1177)抗体用于检测内皮细胞中磷酸化水平，证实其与NO释放的正相关性。
3. **"Reciprocal regulation of endothelial nitric oxide synthase by Ca2+-calmodulin and caveolin"**
- 作者：Michel JB, et al.
- 摘要：探讨eNOS活性受钙调蛋白和窖蛋白(caveolin)的双向调控，S1177磷酸化可拮抗窖蛋白抑制作用。研究通过该抗体证明磷酸化修饰在病理生理条件下的动态变化。
4. **"Phosphorylation of eNOS at Ser1179 in hypoxia-induced pulmonary hypertension"**
- 作者：Fagan KA, et al.
- 摘要：分析缺氧性肺动脉高压模型中eNOS S1177(小鼠为S1179)磷酸化水平的变化，使用特异性抗体发现磷酸化减少与内皮功能障碍相关，提示其病理意义。
这些文献均利用Phospho-eNOS(S1177)抗体揭示了该位点磷酸化在NO信号通路及心血管疾病中的调控机制。
The Phospho-eNOS(S1177) antibody is a key tool for studying the activation status of endothelial nitric oxide synthase (eNOS), a critical enzyme regulating vascular homeostasis. eNOS, encoded by the *NOS3* gene, catalyzes nitric oxide (NO) production, which maintains vascular tone, endothelial function, and blood flow. Its activity is tightly regulated by post-translational modifications, particularly phosphorylation at serine 1177 (S1177 in humans; S1176 in mice). This phosphorylation event, mediated by kinases such as Akt/PKB in response to stimuli like shear stress or insulin, enhances eNOS enzymatic activity and NO release.
The Phospho-eNOS(S1177) antibody specifically detects eNOS when phosphorylated at this residue, enabling researchers to assess its activation in physiological or pathological contexts. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study endothelial dysfunction in cardiovascular diseases (e.g., hypertension, atherosclerosis), diabetes, or preeclampsia. By distinguishing phosphorylated (active) eNOS from its unmodified form, this antibody helps elucidate signaling pathways (e.g., PI3K/Akt) and therapeutic targets aimed at improving NO-dependent vasodilation. Validation across species and sample types (cells, tissues) ensures its utility in both basic research and drug development studies focused on vascular health.
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