| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Chaperone activity of bc1 complex-like, mitochondrial, Chaperone-ABC1-like, 2711-, aarF domain-containing protein kinase 3, ADCK3, CABC1 |
| Entrez GeneID | 56997 |
| WB Predicted band size | 72.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CABC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human CABC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于CABC1(C-terminal D531)抗体的示例参考文献(注：部分文献为假设性示例，实际引用时需核实)：
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1. **文献名称**: *"CABC1 regulates mitochondrial complex assembly through its C-terminal domain"*
**作者**: Müller, J. et al.
**摘要**: 本研究利用针对CABC1 C端D531位点的特异性抗体，通过免疫共沉淀和蛋白质组学分析，揭示了CABC1在线粒体复合物III组装中的关键作用，并证明其缺失导致氧化磷酸化功能障碍。
2. **文献名称**: *"A novel antibody targeting CABC1-D531 reveals tissue-specific expression patterns"*
**作者**: Sato, K. et al.
**摘要**: 作者开发了一种高特异性CABC1(D531)抗体，通过免疫组化验证其在心脏和肝脏中的高表达，并发现其表达水平与代谢性疾病模型中的线粒体损伤相关。
3. **文献名称**: *"CABC1 phosphorylation at D531 modulates cellular stress responses"*
**作者**: Chen, L. et al.
**摘要**: 研究利用C-terminal D531抗体证实，CABC1在氧化应激条件下的磷酸化修饰影响其与HSP70的相互作用，进而调控细胞凋亡通路。
4. **文献名称**: *"Validation of a commercial CABC1-D531 antibody for diagnostic applications"*
**作者**: Gupta, R. et al.
**摘要**: 文章系统评估了市售CABC1(D531)抗体的灵敏度和特异性，证明其在Western blot和免疫荧光中的可靠性，并用于遗传性线粒体疾病的临床样本检测。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Google Scholar或抗体供应商提供的参考文献链接获取真实文献。若需进一步协助定位具体文献，请提供更多研究背景或抗体货号信息。
The CABC1 (C-term D531) antibody is a polyclonal antibody targeting the C-terminal region of the CABC1 protein, specifically around residue aspartate 531 (D531). CABC1. also known as COQ8B or ADCK3. is a mitochondrial kinase-like protein critical for the biosynthesis of coenzyme Q (CoQ10), a vital component of the electron transport chain and cellular antioxidant defense. Mutations in the CABC1 gene are linked to CoQ10 deficiency disorders, causing severe neurological and muscular pathologies.
This antibody is commonly used in research to detect and quantify endogenous CABC1 protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. It is often raised in rabbits using a synthetic peptide corresponding to the C-terminal sequence of human CABC1. ensuring specificity for the target epitope. Validation typically includes knockout controls or siRNA-mediated knockdown to confirm minimal cross-reactivity.
The C-terminal region recognized by the antibody is implicated in CABC1’s interaction with other CoQ biosynthesis enzymes and its putative kinase activity, which regulates CoQ10 synthesis. Studying CABC1 using this antibody aids in understanding mitochondrial dysfunction mechanisms, CoQ10-related diseases, and potential therapeutic strategies. Its application spans cell biology, neurodegenerative disease research, and metabolic disorder studies, making it a valuable tool for dissecting CABC1’s molecular roles.
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