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Rabbit Polyclonal DLG7(N-term) Antibody

  • 中文名: DLG7 (N-term)抗体
  • 别    名: Disks large-associated protein 5, DAP-5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein, HURP, DLGAP5, DLG7, KIAA0008
货号: IPDX30330
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDisks large-associated protein 5, DAP-5, Discs large homolog 7, Disks large-associated protein DLG7, Hepatoma up-regulated protein, HURP, DLGAP5, DLG7, KIAA0008
Entrez GeneID9787
WB Predicted band size95.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis DLG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DLG7.
FormulationPurified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

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参考文献

以下是关于DLG7 (N-term)抗体的模拟参考文献示例(仅供参考,具体文献需通过学术数据库检索确认):

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1. **文献名称**:*DLG7 Expression in Mitotic Progression: Role of N-terminal Domain in Spindle Pole Localization*

**作者**:Zhang Y, et al.

**摘要**:该研究使用DLG7 (N-term)抗体进行免疫荧光染色,发现DLG7的N端结构域对定位到有丝分裂纺锤体极体至关重要,敲低DLG7导致染色体排列异常,提示其在细胞分裂中的功能依赖性。

2. **文献名称**:*DLG7 as a Prognostic Marker in Ovarian Cancer: Immunohistochemical Analysis*

**作者**:Chen L, et al.

**摘要**:通过DLG7 (N-term)抗体的免疫组化实验,证实DLG7在卵巢癌组织中高表达,且与患者生存率负相关,提示其可能作为癌症治疗的潜在靶点。

3. **文献名称**:*Characterization of DLG7 Antibody Specificity Using CRISPR-Cas9 Knockout Validation*

**作者**:Abcam Technical Report

**摘要**:该技术报告验证了DLG7 (N-term)抗体的特异性,通过CRISPR敲除DLG7的细胞系进行Western blot和免疫沉淀实验,证明抗体在多种实验条件下的可靠性。

4. **文献名称**:*DLG7 Interaction with Aurora Kinase B in Regulating Mitotic Checkpoint*

**作者**:Wang H, et al.

**摘要**:利用DLG7 (N-term)抗体进行共免疫沉淀实验,发现DLG7与Aurora激酶B在维持有丝分裂检查点中的相互作用,为靶向治疗提供了分子机制依据。

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**建议**:实际文献可通过PubMed、Google Scholar或抗体供应商(如CST、Abcam)的“引用文献”板块检索,关键词包括“DLG7 antibody”、“DLG7 N-terminal”或“DLG7 mitosis”。

背景信息

The DLG7 (N-term) antibody targets the N-terminal region of DLG7 (Discs Large Homolog 7), a protein critical for mitotic progression and spindle formation. DLG7. also known as hDLG7 or SAP-97-like protein, belongs to the membrane-associated guanylate kinase (MAGUK) family, characterized by PDZ, SH3. and GUK domains. It plays a key role in maintaining spindle integrity, regulating chromosome alignment, and ensuring accurate segregation during cell division. The N-terminal region of DLG7 contains conserved domains essential for protein-protein interactions, particularly during cytokinesis and checkpoint signaling. Dysregulation of DLG7 is linked to genomic instability and cancer progression, with overexpression observed in various malignancies. The DLG7 (N-term) antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its localization, expression dynamics, and functional roles in cell cycle regulation. Its specificity for the N-terminus makes it valuable for distinguishing DLG7 isoforms and investigating post-translational modifications or binding partners in mitotic machinery. Research applications span cancer biology, mitotic dysfunction studies, and therapeutic target validation.

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