| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CER1 |
| Uniprot No | O95813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-267aa |
| 氨基酸序列 | TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTS PAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPID GMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPF SQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTM HLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA VDHHHHHH |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CER1重组蛋白的3篇参考文献摘要简述：
1. **文献名称**：*Cerberus1重组蛋白在干细胞分化中的调控作用*
**作者**：Smith A, et al.
**摘要**：研究通过重组CER1蛋白处理人胚胎干细胞，证实其通过抑制Nodal信号通路促进神经外胚层分化，为再生医学提供潜在靶点。
2. **文献名称**：*重组CER1蛋白与TGF-β超家族配体的相互作用分析*
**作者**：Zhang L, et al.
**摘要**：体外实验表明，重组CER1蛋白可选择性结合Activin和BMP4.阻断下游Smad磷酸化，揭示其在胚胎发育中的多重信号调控机制。
3. **文献名称**：*基于HEK293细胞的高效CER1重组表达系统构建*
**作者**：Wang Y, et al.
**摘要**：优化CER1基因密码子并利用哺乳动物表达系统实现可溶性重组蛋白的高产，纯化产物经质谱验证具备天然抑制活性。
注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science以“CER1 recombinant protein”为关键词检索最新研究。
CER1 (Cerberus 1) is a secreted protein belonging to the Cerberus/DAN family, known for its role in embryonic development and tissue homeostasis. It functions as a multifunctional signaling antagonist, primarily inhibiting key developmental pathways such as Nodal, BMP (bone morphogenetic protein), and Wnt. These pathways regulate cell differentiation, body axis formation, and organogenesis. CER1 is notably expressed during early embryogenesis, particularly in the anterior visceral endoderm and primitive streak, where it helps establish embryonic patterning by fine-tuning signaling gradients. Its ability to modulate multiple pathways makes it critical for balancing cellular responses during development.
Structurally, CER1 contains a conserved cysteine-rich domain, forming a characteristic cystine-knot motif that stabilizes its interactions with signaling molecules. Recombinant CER1 protein is engineered using expression systems like mammalian cells or bacteria to produce bioactive forms for research and therapeutic applications. Researchers utilize it to study developmental biology, stem cell differentiation, and tissue regeneration. In regenerative medicine, CER1 has shown potential in promoting tissue repair and mitigating fibrosis by suppressing pro-fibrotic BMP signaling. Additionally, its role in cancer is being explored, as dysregulation of CER1-linked pathways is implicated in tumor progression and metastasis.
The development of recombinant CER1 has advanced disease modeling, organoid culture, and drug screening. Its application extends to studying congenital disorders and designing targeted therapies. However, challenges remain in optimizing its stability, delivery, and specificity in vivo. Ongoing research aims to harness CER1's regulatory properties for clinical interventions in developmental abnormalities, degenerative diseases, and cancer.
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