| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX |
| Entrez GeneID | 8575 |
| WB Predicted band size | 34.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PRKRA Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human PRKRA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Phosphorylation of PRKRA at Serine 246 Modulates Innate Immune Signaling"**
- **Authors**: Lee et al.
- **摘要**: 研究揭示了PRKRA在抗病毒反应中S246位点的磷酸化通过调控RIG-I信号通路增强I型干扰素产生的机制，抗体用于验证激酶PKCδ介导的磷酸化作用。
2. **"PRKRA Phosphorylation in Stress Granule Dynamics"**
- **Authors**: Smith et al.
- **摘要**: 利用Phospho-PRKRA(S246)抗体证实氧化应激下S246磷酸化促进PRKRA与应激颗粒蛋白的相互作用，影响细胞应激反应的调控。
3. **"Role of PRKRA in Parkinson’s Disease Pathogenesis"**
- **Authors**: Chen et al.
- **摘要**: 通过该抗体检测到帕金森病患者细胞模型中PRKRA(S246)磷酸化水平异常，提示其与神经炎症及线粒体功能障碍相关。
4. **"Kinase-Specific Regulation of PRKRA in Cancer Metastasis"**
- **Authors**: Wang et al.
- **摘要**: 研究证明Aurora激酶对PRKRA(S246)的磷酸化促进肿瘤细胞侵袭，抗体用于评估磷酸化状态与临床预后的相关性。
(注：以上文献为虚拟示例，实际研究中建议通过PubMed或Google Scholar检索具体抗体引用文献。)
The Phospho-PRKRA(S246) antibody is designed to detect the phosphorylation of protein kinase, interferon-inducible double-stranded RNA-dependent activator (PRKRA, also known as PACT) at serine residue 246. PRKRA is a critical regulatory protein involved in innate immune responses, particularly in antiviral defense and cellular stress signaling. It interacts with the double-stranded RNA-activated protein kinase (PKR), forming a complex that activates PKR in response to viral infection or stress stimuli. This activation triggers downstream pathways, including eIF2α phosphorylation, leading to translational inhibition and stress granule formation.
Phosphorylation at Ser246 is a post-translational modification that modulates PRKRA’s ability to regulate PKR activity and its own functional interactions. Studies suggest this modification may influence PRKRA’s role in stress response, apoptosis, and autophagy. The Phospho-PRKRA(S246) antibody is widely used in research to investigate the dynamic regulation of PRKRA under conditions such as viral infection, oxidative stress, or endoplasmic reticulum stress. It is validated for applications like Western blotting, immunoprecipitation, and immunofluorescence to assess PRKRA activation states. Dysregulation of PRKRA phosphorylation has been implicated in diseases such as cancer and neurodegenerative disorders, making this antibody a valuable tool for exploring molecular mechanisms in pathogenesis and therapeutic targeting.
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