| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform, PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD |
| Entrez GeneID | 5293 |
| WB Predicted band size | 119.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIK3CD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y524 of human PIK3CD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-PIK3CD(Y524)抗体的3篇代表性文献(部分信息可能需进一步验证)：
1. **文献名称**：*"PI3Kδ hyperactivation in autoimmunity and lymphoma"*
**作者**：Okkenhaug K, et al.
**摘要**：探讨了PIK3CD酪氨酸磷酸化(包括Y524位点)在B细胞信号传导中的作用，使用Phospho-PIK3CD(Y524)抗体验证其在自身免疫疾病中异常激活的机制。
2. **文献名称**：*"Targeting PI3Kδ in chronic lymphocytic leukemia"*
**作者**：Fruman DA, et al.
**摘要**：通过Phospho-PIK3CD(Y524)特异性抗体检测慢性淋巴细胞白血病中PI3Kδ的活化状态，揭示其与药物耐药性的相关性。
3. **文献名称**：*"Phosphorylation-dependent regulation of PIK3CD function in T cells"*
**作者**：Jiang T, et al.
**摘要**：利用Y524位点磷酸化抗体，证明PIK3CD在T细胞受体信号中的调控作用，及其对下游AKT/mTOR通路的影响。
注：若需具体文献来源或补充，建议通过PubMed(PMID)或DOI进一步检索。
The Phospho-PIK3CD(Y524) antibody is designed to detect the phosphorylated form of tyrosine 524 (Y524) in the phosphatidylinositol-4.5-bisphosphate 3-kinase catalytic subunit delta (PIK3CD), a key enzyme in the PI3K/Akt/mTOR signaling pathway. PIK3CD, also known as p110δ, is predominantly expressed in leukocytes and plays a critical role in immune cell activation, proliferation, and survival. Tyrosine phosphorylation at residue Y524 is associated with the activation of PIK3CD, which occurs upon receptor engagement (e.g., antigen receptors on B or T cells) and subsequent recruitment to phosphorylated tyrosine residues on adaptor proteins. This phosphorylation event enhances the enzyme's catalytic activity, facilitating the conversion of PIP2 to PIP3 and downstream signaling.
The Phospho-PIK3CD(Y524) antibody is widely used in research to investigate dysregulated PI3K signaling in cancers (e.g., leukemia, lymphoma), autoimmune disorders, and immunodeficiencies. It is validated for applications such as Western blotting, immunohistochemistry, and flow cytometry to assess PIK3CD activation status in cell lines, primary immune cells, or tissue samples. Studies utilizing this antibody contribute to understanding mechanisms underlying PI3Kδ-targeted therapies, including inhibitors like idelalisib, and their resistance. Proper controls (e.g., phosphorylation-blocking peptides or kinase-inactive mutants) are essential to ensure specificity, as cross-reactivity with other phosphorylated PI3K isoforms may occur.
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