| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2 |
| Entrez GeneID | 4204 |
| WB Predicted band size | 52.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from human MeCP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MeCP2抗体相关的文献示例(内容基于学术研究背景整理，非实时检索结果)：
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1. **文献名称**: *"Antibody-based validation of CNS-specific MeCP2 protein expression in Rett syndrome"*
**作者**: Nan X, Tate P, Li E, et al.
**摘要**: 本研究验证了多种MeCP2抗体在脑组织中的特异性，通过Western blot和免疫组化证明其在Rett综合征小鼠模型中能特异性识别MeCP2蛋白，并确认抗体在人类和小鼠组织中的交叉反应性。
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2. **文献名称**: *"Comparative analysis of commercial MeCP2 antibodies for chromatin immunoprecipitation (ChIP) assays"*
**作者**: Skene PJ, Henikoff S.
**摘要**: 文章对比了5种市售MeCP2抗体在ChIP实验中的表现，发现部分抗体存在非特异性结合，并推荐了两种在染色质结合研究中表现稳定的抗体，强调了抗体验证对表观遗传研究的重要性。
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3. **文献名称**: *"Epitope mapping of MeCP2 antibodies reveals variability in detecting phosphorylation-dependent isoforms"*
**作者**: Tillotson R, Selfridge J, Koerner MV, et al.
**摘要**: 通过表位定位分析，揭示了不同MeCP2抗体对磷酸化修饰亚型的检测差异，提出需根据研究目的(如检测全长蛋白或特定修饰形式)选择特异性抗体，并提供了抗体筛选的实验方案。
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**注**：以上文献信息为模拟示例，实际引用请通过PubMed或Google Scholar检索关键词(如"MeCP2 antibody validation")获取最新论文。如需具体文献，可补充提供研究方向(如抗体应用场景或疾病模型)，我将进一步优化推荐内容。
The MeCP2 (methyl-CpG-binding protein 2) antibody is a crucial tool in neuroscience and epigenetics research, targeting the MeCP2 protein encoded by the *MECP2* gene. MeCP2 binds methylated DNA regions, acting as a transcriptional regulator by recruiting chromatin-modifying complexes. It plays a vital role in neuronal development, synaptic plasticity, and maintaining chromatin structure. Mutations in *MECP2* are linked to Rett syndrome, a severe neurodevelopmental disorder, and *MECP2* duplication syndrome, highlighting its importance in brain function.
MeCP2 antibodies are widely used to detect protein expression, localization, and post-translational modifications (e.g., phosphorylation) in tissues and cell lines. Applications include Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP). Researchers prioritize antibodies validated for specificity across species (human, mouse, rat) and compatibility with distinct MeCP2 isoforms (e.g., MeCP2-E1/E2). Quality control often involves testing in *MECP2* knockout models or siRNA-treated samples to confirm target specificity.
These antibodies are indispensable for studying MeCP2’s role in neurodevelopmental disorders, autism spectrum conditions, and epigenetic mechanisms. They also support therapeutic research, such as evaluating gene therapy efficacy or small-molecule interventions targeting MeCP2 dysfunction. However, variability in antibody performance (e.g., cross-reactivity, batch consistency) necessitates careful validation to ensure experimental reproducibility.
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