| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CCND3 |
| Entrez GeneID | 896 |
| clone | 6H4 |
| WB Predicted band size | 32kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human CCND3 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Phospho-LC3C(S12)抗体** 的参考文献示例(内容为虚构，供参考格式)：
1. **《LC3C Serine 12 phosphorylation regulates selective autophagy in viral defense》**
- **作者**: Wild P et al.
- **摘要**: 本研究开发了特异性识别LC3C S12磷酸化位点的抗体，证实该修饰在抗病毒自噬(如xenophagy)中通过增强自噬体与病原体的结合发挥关键作用。
2. **《Phosphorylation-dependent regulation of LC3 isoforms in autophagy initiation》**
- **作者**: Ichimura Y et al.
- **摘要**: 通过Phospho-LC3C(S12)抗体的应用，揭示了LC3C与其他亚型(如LC3B)的磷酸化差异，表明S12位点磷酸化可能通过招募特定接头蛋白调控自噬体成熟。
3. **《A novel role for LC3C phosphorylation in cancer cell chemoresistance》**
- **作者**: Zhang X et al.
- **摘要**: 利用Phospho-LC3C(S12)抗体检测发现，化疗药物诱导的LC3C S12磷酸化通过激活非经典自噬通路促进肿瘤细胞存活，为耐药性机制提供了新靶点。
4. **《Antibody-based profiling of autophagy-related phosphorylation events》**
- **作者**: Noda NN et al.
- **摘要**: 系统评估了多种自噬相关抗体的特异性，包括Phospho-LC3C(S12)，确认其在人源细胞和小鼠模型中的可靠性，并应用于高通量自噬通量分析。
**注**：以上文献为示例，实际研究中需根据具体数据库(如PubMed、Google Scholar)检索真实发表的论文。若该抗体较新或研究较少，建议查阅相关公司(如CST、Abcam)的产品引用文献列表。
Phospho-LC3C(S12) antibody is a specialized tool used to study the post-translational modification of microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C), a key component of the autophagy pathway. Autophagy, a cellular degradation process, involves the formation of autophagosomes that engulf cytoplasmic components for lysosomal breakdown. LC3 proteins, including LC3C, are critical in autophagosome membrane expansion and cargo recognition.
LC3C, a less-studied isoform compared to LC3A/B, is implicated in selective autophagy processes, such as xenophagy (pathogen clearance) and aggrephagy (protein aggregate removal). Phosphorylation at serine 12 (S12) of LC3C is thought to regulate its interaction with autophagy adaptors or subcellular localization. This modification may influence LC3C’s role in distinct autophagic pathways, potentially linking it to stress responses or pathogen defense mechanisms.
The Phospho-LC3C(S12) antibody detects this specific phosphorylation event, enabling researchers to investigate LC3C activation dynamics in experimental models. It is commonly used in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess autophagy flux or pathway-specific regulation. Studies utilizing this antibody contribute to understanding isoform-specific autophagy mechanisms, particularly in contexts like viral infection, cancer, or neurodegenerative diseases where LC3C’s unique functions are increasingly recognized. Its development underscores the growing interest in deciphering the nuanced roles of LC3 isoforms in health and disease.
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