| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NXPH1 |
| Uniprot No | P58417 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-271aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSANLTNGG KSELLKSGSS KSTLKHIWTE SSKDLSISRL LSQTFRGKEN DTDLDLRYDT PEPYSEQDLW DWLRNSTDLQ EPRPRAKRRP IVKTGKFKKM FGWGDFHSNI KTVKLNLLIT GKIVDHGNGT FSVYFRHNST GQGNVSVSLV PPTKIVEFDL AQQTVIDAKD SKSFNCRIEY EKVDKATKNT LCNYDPSKTC YQEQTQSHVS WLCSKPFKVI CIYISFYSTD YKLVQKVCPD YNYHSDTPYF PSG |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NXPH1重组蛋白的3篇参考文献示例：
1. **"Recombinant production and functional analysis of neurexophilin-1 (NXPH1) in synaptic protein interactions"**
*作者：Miller A, et al.*
摘要：本研究通过昆虫细胞系统表达重组NXPH1蛋白，证实其特异性结合neurexin家族成员，揭示了其在突触细胞粘附中的调控作用。
2. **"Crystal structure of human NXPH1 reveals a conserved neurexin-binding motif"**
*作者：Tanaka R, et al.*
摘要：利用重组NXPH1蛋白解析了其晶体结构，发现其N端结构域含保守结合模体，为阐明其与neurexin互作的分子机制提供结构基础。
3. **"High-efficiency purification of recombinant NXPH1 for in vitro neurite outgrowth assays"**
*作者：Chen L, et al.*
摘要：开发了基于HEK293细胞的重组NXPH1蛋白纯化方案，并证明其在体外能显著增强原代神经元的轴突生长活性。
注：上述文献为示例性内容，实际研究中建议通过PubMed或Web of Science以"NXPH1 recombinant"为关键词检索最新文献。
**Background of NXPH1 Recombinant Protein**
Neurexophilin-1 (NXPH1) is a secreted glycoprotein belonging to the neurexophilin family, which comprises four members (NXPH1-4) in humans. These proteins are characterized by their conserved N-terminal region, a variable middle domain, and a C-terminal region that shares homology with neuropeptide-like motifs. NXPH1 was initially identified as a ligand for neurexins, a family of presynaptic cell adhesion molecules critical for synaptic organization and neurotransmission. The interaction between NXPH1 and neurexins is thought to play a role in modulating synaptic plasticity, neuronal connectivity, and signal transduction at central nervous system (CNS) synapses.
NXPH1 is predominantly expressed in the brain, particularly in inhibitory GABAergic and glycinergic neurons, suggesting its involvement in regulating inhibitory neurotransmission. Studies have linked NXPH1 to neurodevelopmental processes, including synapse formation, stabilization, and circuit refinement. Dysregulation of NXPH1 has been implicated in neurological disorders such as autism spectrum disorders (ASD), schizophrenia, and epilepsy, highlighting its potential as a therapeutic or diagnostic target.
Recombinant NXPH1 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper post-translational modifications, particularly glycosylation, which is crucial for its functional activity. This purified protein serves as a vital tool for in vitro and in vivo studies, enabling researchers to investigate NXPH1’s structural properties, binding partners (e.g., neurexins, α-neurexins), and signaling mechanisms. It also aids in exploring its role in disease models and screening for molecules that modulate its activity.
Overall, NXPH1 recombinant protein provides a valuable resource for advancing our understanding of synaptic biology and developing targeted interventions for neurological conditions.
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