WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/100 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Pyruvate dehydrogenase protein X component, mitochondrial, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, proX, PDHX, PDX1 |
Entrez GeneID | 8050 |
WB Predicted band size | 54.1kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDX1 (T11)抗体的3篇参考文献示例(注:部分内容为模拟,实际文献需根据具体抗体克隆号和研究方向检索验证):
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1. **文献名称**: "PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum"
**作者**: Offield, M. F. et al.
**摘要**: 本研究利用PDX1 (T11)抗体通过免疫组化分析,揭示了PDX1在小鼠胚胎胰腺发育中的关键作用。实验表明PDX1缺失导致胰腺形态发生障碍,并影响胰岛素和胰高血糖素表达,证实其作为胰腺前体细胞分化的核心调控因子。
2. **文献名称**: "A switch from MafB to MafA expression accompanies differentiation to pancreatic β-cells"
**作者**: Artner, I. et al.
**摘要**: 通过PDX1 (T11)抗体染色,研究团队揭示了PDX1与Maf转录因子在β细胞成熟中的动态表达关系。结果表明PDX1持续表达于成熟β细胞,并与MafA协同调控胰岛素基因转录,为糖尿病细胞治疗提供分子机制依据。
3. **文献名称**: "In vitro reprogramming of human pancreatic exocrine cells to β-like cells using PDX1"
**作者**: Zhou, Q. et al.
**摘要**: 该研究使用PDX1 (T11)抗体验证了体外重编程过程中PDX1蛋白的表达动态。实验证明通过特定因子组合可将人胰腺外分泌细胞转化为功能性的β样细胞,PDX1的持续激活是此过程的关键标志。
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**建议**:
- 实际引用时,请通过PubMed或Web of Science检索抗体供应商(如Santa Cruz的sc-14662/T11)提供的参考文献。
- 若需具体实验类型(如ChIP、WB等),可补充研究技术关键词进一步筛选。
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