| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2 |
| Entrez GeneID | 389421 |
| WB Predicted band size | 27.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-250 amino acids from the C-terminal region of human LIN28B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于LIN28B抗体的3篇示例文献(内容为模拟概括，仅供参考)：
1. **文献名称**：*LIN28B regulates stem cell metabolism and pluripotency through antibody-validated protein interactions*
**作者**：Nguyen, T. et al.
**摘要**：研究通过LIN28B特异性抗体验证其在胚胎干细胞中的功能，发现其通过结合RNA和代谢调控蛋白维持多能性，抗体用于Western blot和免疫沉淀实验。
2. **文献名称**：*LIN28B as a prognostic biomarker in cancer: Validation via immunohistochemical analysis*
**作者**：Zhang, Y. et al.
**摘要**：利用商业化LIN28B抗体对多种癌症组织进行免疫组化分析，证实其高表达与患者不良预后相关，并验证抗体在临床样本中的特异性。
3. **文献名称**：*Antibody-based detection of LIN28B in cellular reprogramming and development*
**作者**：Viswanathan, S. et al.
**摘要**：研究通过抗体验证LIN28B在体细胞重编程中的作用，发现其过表达可增强诱导多能干细胞效率，抗体用于流式细胞术和免疫荧光定位。
4. **文献名称**：*LIN28B-mediated oncogenesis and therapeutic targeting in hepatocellular carcinoma*
**作者**：Peng, C. et al.
**摘要**：通过抗体验证LIN28B在肝癌中的表达上调，证明其通过let-7/mTOR通路促进肿瘤生长，抗体用于组织微阵列分析和功能抑制实验。
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**备注**：以上为模拟文献示例，实际引用请通过PubMed、Google Scholar等平台检索关键词“LIN28B antibody”或结合具体研究场景筛选。
LIN28B is an RNA-binding protein involved in regulating gene expression, primarily known for its role in embryonic development, stem cell pluripotency, and cellular reprogramming. It functions by binding to mRNAs or miRNAs, such as let-7 family members, to block their maturation, thereby influencing processes like differentiation, metabolism, and oncogenesis. LIN28B is classified as an oncofetal protein due to its high expression during embryogenesis and re-emergence in various cancers, including neuroblastoma, hepatocellular carcinoma, and germ cell tumors. Its overexpression is often linked to aggressive tumor behavior, metastasis, and poor prognosis.
Antibodies targeting LIN28B are essential tools for studying its expression patterns, subcellular localization, and functional roles in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to validate LIN28B levels in tissues or cell lines. Given LIN28B's potential as a diagnostic or prognostic biomarker, its antibodies also hold translational relevance for cancer research and therapeutic development. However, antibody specificity remains a critical consideration, as LIN28B shares homology with its paralog, LIN28A. Reliable LIN28B-specific antibodies enable researchers to dissect its unique contributions to developmental pathways and disease mechanisms, advancing insights into stem cell biology and oncology.
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