| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NTNG1 |
| Uniprot No | Q9Y2I2-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MYLSRFLSIH ALWVTVSSVM QPYPLVWGHY DLCKTQIYTE EGKVWDYMAC QPESTDMTKY LKVKLDPPDI TCGDPPETFC AMGNPYMCNN ECDASTPELA HPPELMFDFE GRHPSTFWQS ATWKEYPKPL QVNITLSWSK TIELTDNIVI TFESGRPDQM ILEKSLDYGR TWQPYQYYAT DCLDAFHMDP KSVKDLSQHT VLEIICTEEY STGYTTNSKI IHFEIKDRFA FFAGPRLRNM ASLYGQLDTT KKLRDFFTVT DLRIRLLRPA VGEIFVDELH LARYFYAISD IKVRGRCKCN LHATVCVYDN SKLTCECEHN TTGPDCGKCK KNYQGRPWSP GSYLPIPKGT ANTCIPSISS IGTNVCDNEL LHCQNGGTCH NNVRCLCPAA YTGILCEKLR CEEAGSCG |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NTNG1重组蛋白的3篇文献示例(基于公开研究主题模拟，建议通过学术数据库核实原文)：
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1. **文献名称**: *"Recombinant NTNG1 Promotes Axon Outgrowth via Integrin Signaling in Cortical Neurons"*
**作者**: Smith A, et al.
**摘要**: 研究通过重组NTNG1蛋白处理体外培养的皮层神经元，发现其通过激活整合素β1信号通路促进轴突生长，揭示了NTNG1在神经再生中的潜在作用。
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2. **文献名称**: *"Structural Characterization of Recombinant Mouse NTNG1 and Its Interaction with Laminin"*
**作者**: Tanaka K, et al.
**摘要**: 利用哺乳动物表达系统纯化小鼠NTNG1重组蛋白，通过X射线晶体学解析其结构，并证实其与细胞外基质蛋白层粘连蛋白的物理结合，为理解神经突触形成机制提供结构基础。
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3. **文献名称**: *"Altered Expression of NTNG1 in Autism Spectrum Disorder: Functional Analysis Using Recombinant Protein Models"*
**作者**: Lee JH, et al.
**摘要**: 通过构建NTNG1重组蛋白，验证其在自闭症患者脑组织中的表达异常，并发现其缺失导致突触可塑性受损，提示NTNG1可能作为神经发育疾病的生物标志物。
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**注意**：以上内容为模拟示例，具体文献需通过PubMed、Google Scholar等平台以“NTNG1 recombinant protein”为关键词检索获取。
**Background of NTNG1 Recombinant Protein**
Netrin G1 (NTNG1) is a member of the netrin family of secreted and membrane-associated proteins, primarily expressed in the central nervous system (CNS). Unlike classical netrins, NTNG1 lacks the conserved N-terminal laminin domain and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage. It plays critical roles in neuronal development, including axon guidance, synaptic connectivity, and the formation of neural circuits. NTNG1 interacts with specific receptors, such as netrin-G ligand (NGL) family proteins, to mediate cell-cell adhesion and signaling processes essential for maintaining synaptic plasticity and neurotransmission.
Dysregulation of NTNG1 has been implicated in neurodevelopmental and neuropsychiatric disorders, including epilepsy, schizophrenia, and autism spectrum disorders. Studies suggest that NTNG1 mutations or altered expression may disrupt neural network assembly, contributing to cognitive and behavioral deficits.
Recombinant NTNG1 protein is engineered in vitro using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and functional activity. It typically includes the extracellular domain to mimic native interactions. This protein serves as a vital tool for studying NTNG1-mediated pathways, screening therapeutic compounds, and exploring mechanisms underlying neurological diseases. Applications range from in vitro binding assays and neuronal cell culture studies to in vivo models for validating therapeutic targets. Its production enables researchers to dissect NTNG1's role in health and disease, offering potential insights for developing biomarkers or targeted therapies for CNS disorders.
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