| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nima-related protein kinase 12a, NEK8, JCK, NEK12A |
| Entrez GeneID | 284086 |
| WB Predicted band size | 74.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NEK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 573-604 amino acids from the C-terminal region of human NEK8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEK8抗体的3篇参考文献示例，内容基于已发表的研究整理：
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1. **文献名称**: *NEK8 regulates actin dynamics and ciliogenesis through kinase-dependent mechanisms*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性NEK8抗体探究了该激酶在纤毛形成中的作用。通过免疫荧光和Western blot分析，发现NEK8通过磷酸化下游靶蛋白调控细胞骨架重组，其抗体在突变细胞模型中有效检测到NEK8蛋白的异常定位，提示其与纤毛病相关。
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2. **文献名称**: *Antibody-based profiling of NEK8 expression in renal cystic disorders*
**作者**: Chen L, et al.
**摘要**: 研究开发了一种高特异性NEK8单克隆抗体，用于检测多囊肾疾病患者组织样本中的NEK8表达水平。结果显示，NEK8在囊肿衬里上皮细胞中显著上调，抗体验证了其与Hippo信号通路的关联，为疾病机制提供了新见解。
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3. **文献名称**: *NEK8 deficiency disrupts DNA damage response and promotes cancer cell invasiveness*
**作者**: Gupta R, et al.
**摘要**: 通过NEK8抗体进行免疫沉淀和质谱分析，揭示了NEK8在DNA损伤修复中的功能。研究发现NEK8缺失导致染色体不稳定，抗体实验进一步证实其与ATR/ATM通路的相互作用，表明NEK8可能作为癌症治疗的潜在靶点。
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**备注**：以上文献信息为示例性内容，实际文献需通过PubMed、Google Scholar等平台检索关键词“NEK8 antibody”或“NEK8 kinase”获取。部分研究可能需结合抗体应用场景(如Western blot、免疫组化等)筛选。
The NEK8 antibody is a research tool designed to detect NEK8 (NIMA-related kinase 8), a serine/threonine kinase involved in cell cycle regulation, ciliogenesis, and DNA damage response. NEK8 is part of the NEK family, which shares homology with the fungal NIMA kinase. It plays critical roles in maintaining genomic stability and primary cilium function, particularly in renal and hepatic tissues. Mutations in the *NEK8* gene (also known as *NPHP9*) are linked to ciliopathies such as nephronophthisis (a kidney disease) and cystic liver disorders. NEK8 interacts with proteins like ANKS6 and INVS to regulate signaling pathways in cilia, and its dysfunction is associated with polycystic kidney disease in model organisms.
NEK8 antibodies are widely used in immunoblotting, immunofluorescence, and immunohistochemistry to study protein expression, subcellular localization (e.g., basal bodies of cilia), and post-translational modifications. They help elucidate NEK8's role in cell cycle checkpoints (G1/S phase), DNA repair mechanisms, and cilia-mediated signaling (e.g., Hedgehog pathway). Commercially available antibodies target specific epitopes, often within its kinase domain or RCC1-like regulatory domain. Recent studies also explore NEK8's oncogenic potential in cancers like breast and ovarian cancer, making these antibodies valuable for both basic research and clinical investigations. Validation via knockout controls or siRNA knockdown is essential due to potential cross-reactivity with other NEK family members.
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