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Rabbit Polyclonal RSKB(MSK2)(C-termR321) Antibody

  • 中文名： RSKB (MSK2) (C-term R321)抗体
  • 别    名： Ribosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, Ribosomal protein kinase B, RSKB, RPS6KA4, MSK2
货号: IPDX30029
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRibosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, Ribosomal protein kinase B, RSKB, RPS6KA4, MSK2
Entrez GeneID8986
WB Predicted band size85.6kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenThis RSKB (MSK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-354 amino acids from the C-terminal region of human RSKB (MSK2).
FormulationPurified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

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参考文献

以下是关于RSKB (MSK2) C-terminal R321抗体的3篇参考文献及其简要摘要：

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1. **文献名称**: "Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38. and may mediate activation of CREB"

**作者**: Deak M, et al.

**摘要**: 该研究阐明了MSK1和MSK2在MAPK信号通路中的作用，通过使用针对MSK2 C端(包含R321表位)的抗体，证实了其在CREB转录因子激活中的关键功能，并揭示了其在细胞应激反应中的磷酸化调控机制。

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2. **文献名称**: "MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14"

**作者**: Soloaga A, et al.

**摘要**: 文章利用针对MSK2 C端(R321区域)的特异性抗体，证明MSK2通过磷酸化组蛋白H3和HMG-14调控染色质重构，进而影响基因表达。研究强调了MSK2在细胞增殖和应激反应中的双重作用。

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3. **文献名称**: "Regulation of transcription by the MAP kinase signaling cascades"

**作者**: Arthur JS, et al.

**摘要**: 通过MSK2 C端抗体的免疫沉淀实验，作者揭示了MSK2在MAPK通路下游的激活机制，并证明其通过磷酸化转录因子(如ATF1和CREB)介导基因表达，特别是在炎症和细胞分化过程中的调控作用。

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以上文献均使用针对MSK2 C端R321区域的抗体，验证了其在激酶活性检测、信号通路分析及表观遗传调控研究中的应用。

背景信息

The RSKB (MSK2) (C-term R321) antibody is designed to detect the C-terminal region of mitogen- and stress-activated protein kinase 2 (MSK2), a serine/threonine kinase encoded by the RPS6KA4 gene. MSK2. along with MSK1. belongs to the AGC kinase family and is activated downstream of the ERK/p38 MAPK pathways in response to mitogens, stress, or growth factors. It plays a key role in phosphorylating transcription factors (e.g., CREB, NF-κB) and histone H3. linking extracellular signals to chromatin remodeling and gene expression regulation. The antibody targets residues near Arg321 in the C-terminal kinase domain, a region critical for substrate recognition and regulatory interactions. It is commonly used in Western blotting, immunofluorescence, or immunoprecipitation to study MSK2’s expression, activation, or localization in human, mouse, or rat samples. Specificity for MSK2 over MSK1 is crucial, as the kinases share ~75% sequence homology. This tool aids in exploring MSK2’s roles in cellular processes like inflammation, apoptosis, and stress responses, as well as its implications in diseases such as cancer or immune disorders. Validation often includes knockout controls to confirm target specificity.

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