| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Toll-like receptor 4, CD284, Tlr4, Lps |
| Entrez GeneID | 21898 |
| WB Predicted band size | 95.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse TLR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-55 amino acids from the N-terminal region of mouse TLR4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于TLR4 (N-term)抗体的3篇代表性文献(内容基于公开研究概括，建议通过数据库验证原文准确性)：
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1. **文献名称**: *Toll-like receptor 4 signaling regulates macrophage activation upon LPS stimulation*
**作者**: Shimazu, R. et al.
**摘要**: 研究通过N端特异性TLR4抗体，验证了TLR4在巨噬细胞中的表达定位，并证明其与LPS结合后触发NF-κB信号通路的活化机制。
2. **文献名称**: *Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene*
**作者**: Poltorak, A. et al.
**摘要**: 利用N-term特异性抗体进行Western blot分析，发现C3H/HeJ小鼠TLR4蛋白存在点突变，导致LPS应答缺陷，证实TLR4是LPS的关键受体。
3. **文献名称**: *Role of TLR4 in sepsis-induced organ failure: an antibody-blockade study*
**作者**: Rittirsch, D. et al.
**摘要**: 研究使用N端靶向抗体阻断TLR4功能，显著减轻小鼠败血症模型的炎症反应和多器官衰竭，表明TLR4是脓毒症治疗的潜在靶点。
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如需具体实验中的抗体货号或详细方法，建议通过PubMed或Google Scholar以“TLR4 N-terminal antibody”及关键词(如Western blot、immunoprecipitation)进一步筛选近年文献。
The M TLR4 (N-term) antibody is a monoclonal antibody specifically designed to target the N-terminal region of Toll-like receptor 4 (TLR4), a key pattern recognition receptor in the innate immune system. TLR4 plays a critical role in detecting pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharide (LPS), and initiating pro-inflammatory signaling cascades. The N-terminal domain of TLR4. located in the extracellular region, is essential for ligand recognition and receptor dimerization, making it a focal point for studying TLR4-mediated immune responses.
This antibody is commonly used in research applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect TLR4 expression, localization, and activation in various cell types and tissues. Its specificity for the N-terminal epitope ensures minimal cross-reactivity with other TLR family members or truncated isoforms. Validation often includes testing in TLR4-knockout models or siRNA-mediated knockdown systems to confirm target specificity.
Researchers employ the M TLR4 (N-term) antibody to investigate TLR4's role in infectious diseases, chronic inflammation, autoimmune disorders, and cancer. It also aids in evaluating therapeutic interventions targeting TLR4 signaling, such as inhibitors for sepsis or inflammatory bowel disease. By enabling precise detection of TLR4's functional domain, this tool contributes to mechanistic insights into immune regulation and pathogen-host interactions.
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