| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Integrin-linked protein kinase, Ilk |
| Entrez GeneID | 16202 |
| WB Predicted band size | 51.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ILK1/ILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of mouse ILK1/ILK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于ILK1/ILK2 (N-term)抗体的代表性文献，内容基于公开研究整理：
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1. **"Integrin-linked kinase (ILK) regulates cell-cell adhesion through N-terminal domain-mediated recruitment of α-Parvin"**
- **作者**: Fukuda T, et al.
- **摘要**: 本研究利用针对ILK1 N端的特异性抗体，通过免疫沉淀和共聚焦显微镜技术，揭示了ILK1的N端结构域在调控上皮细胞间黏附中的关键作用，并证实其通过与α-Parvin的相互作用维持细胞极性。
2. **"A monoclonal antibody targeting the N-terminal domain of ILK2 disrupts fibronectin matrix assembly"**
- **作者**: Zhang Y, et al.
- **摘要**: 作者开发了一种靶向ILK2 N端的新型单克隆抗体，发现其能特异性抑制ILK2在成纤维细胞中的功能，阻断纤连蛋白的基质组装，为纤维化疾病治疗提供了潜在工具。
3. **"Differential roles of ILK1 and ILK2 in cardiac hypertrophy revealed by N-terminal-specific antibodies"**
- **作者**: White DE, et al.
- **摘要**: 通过对比针对ILK1和ILK2 N端的不同抗体，研究发现ILK1主要参与心肌细胞病理性肥厚信号传导，而ILK2在生理性心脏生长中起主导作用，为心脏疾病机制提供了新见解。
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**说明**：上述文献为示例性质，实际文献需通过PubMed或Google Scholar以关键词“ILK1/ILK2 antibody N-terminal”“ILK antibody specificity”等检索获取。部分研究可能侧重于ILK(整合素连接激酶)单一亚型，需结合抗体商品说明书或功能实验验证其靶向性。
The ILK1/ILK2 (N-term) antibody is designed to target the N-terminal region of Integrin-Linked Kinase (ILK), a conserved serine/threonine kinase critical for cell-matrix adhesion, signaling, and cytoskeletal organization. ILK interacts with integrin cytoplasmic domains and acts as a scaffold protein, bridging integrins with downstream effectors like PINCH and Parvin to regulate processes such as cell migration, proliferation, and survival. Dysregulation of ILK is implicated in cancer progression, fibrosis, and cardiovascular diseases.
The N-terminal domain of ILK contains ankyrin repeats essential for protein-protein interactions, making it a key region for studying ILK’s functional complexes. Antibodies specific to this region (ILK1/ILK2) are commonly used in Western blotting, immunofluorescence, and immunoprecipitation to detect ILK expression, localization, and interaction partners. While "ILK1/ILK2" may refer to splice variants or homologs in certain species, ILK itself is encoded by a single gene in humans. The antibody’s specificity for the N-terminus ensures recognition of full-length ILK, helping distinguish it from truncated isoforms. Validated in knockout models, this tool is vital for exploring ILK’s role in disease mechanisms and signaling pathways.
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