| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1LC3A |
| Uniprot No | Q9H492 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-120aa |
| 氨基酸序列 | MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVSVSTPIADIYE QEKDEDGFLY MVYASQETFG LEHHHHHH |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP1LC3A重组蛋白的3篇参考文献示例(内容基于公开研究归纳，非真实文献)：
1. **《LC3. a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing》**
- 作者：Kabeya, Y., et al.
- 摘要：研究首次描述了MAP1LC3A(LC3)蛋白在自噬过程中的作用，通过重组蛋白表达验证了LC3-I向LC3-II的脂化修饰，并证明其在自噬体膜上的定位，为自噬检测提供了标志物。
2. **《Guidelines for the use and interpretation of assays for monitoring autophagy》**
- 作者：Klionsky, D.J., et al.
- 摘要：指南中总结了LC3重组蛋白在自噬研究中的应用，包括免疫印迹、荧光标记等方法，强调重组LC3蛋白在体外自噬活性分析中的重要性。
3. **《SQSTM1/p62 regulates the expression of LC3 through interaction with the N-terminal region of LC3》**
- 作者：Shibutani, S.T., et al.
- 摘要：利用重组MAP1LC3A蛋白进行体外结合实验，揭示了LC3与SQSTM1/p62蛋白的相互作用机制，阐明其在选择性自噬底物识别中的功能。
如需具体文献，建议通过PubMed或Google Scholar以“MAP1LC3A recombinant protein”“LC3 autophagy”等关键词检索近年论文。
MAP1LC3A (microtubule-associated protein 1 light chain 3 alpha) is a key protein involved in autophagy, a conserved cellular process responsible for degrading and recycling cytoplasmic components. As a mammalian homolog of yeast Atg8. LC3A plays a critical role in autophagosome formation, where it is conjugated to phosphatidylethanolamine (PE) during autophagy initiation. This lipidated form, LC3-II, integrates into autophagosomal membranes, serving as a marker for tracking autophagic activity. Dysregulation of LC3A-mediated autophagy is linked to various diseases, including cancer, neurodegenerative disorders, and infectious diseases, making it a focal point in biomedical research.
Recombinant MAP1LC3A protein is engineered using expression systems like *E. coli* or mammalian cell cultures to produce purified, functional LC3A for experimental applications. Its recombinant form retains the ability to undergo post-translational modifications (e.g., lipidation) when expressed in mammalian systems, mimicking native behavior. Researchers often tag the protein with fluorescent markers (e.g., GFP) or affinity tags (e.g., His-tag) to facilitate visualization, purification, or interaction studies.
This recombinant tool is widely utilized to investigate autophagy mechanisms, screen autophagy-modulating drugs, and quantify autophagic flux in cellular models. In disease research, it aids in exploring how autophagy contributes to tumor progression, pathogen clearance, or neuronal survival. Additionally, LC3A puncta formation assays using recombinant proteins or transfected constructs remain a gold standard for autophagy monitoring. Its versatility and functional relevance underscore its importance in elucidating autophagy-related pathways and developing therapeutic strategies targeting autophagy dysregulation.
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