| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCG2 |
| Uniprot No | P13521 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 418-617aa |
| 氨基酸序列 | TPGRAGTEALPDGLSVEDILNLLGMESAANQKTSYFPNPYNQEKVLPRLPYGAGRSRSNQLPKAAWIPHVENRQMAYENLNDKDQELGEYLARMLVKYPEIINSNQVKRVPGQGSSEDDLQEEEQIEQAIKEHLNQGSSQETDKLAPVSKRFPVGPPKNDDTPNRQYWDEDLLMKVLEYLNQEKAEKGREHIAKRAMENM |
| 预测分子量 | 54.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCG2(Secretogranin II)重组蛋白研究的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: "Expression and Purification of Recombinant Human Secretogranin II in Escherichia coli"
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统高效表达SCG2重组蛋白的优化方法，利用His标签纯化技术获得高纯度蛋白，并验证其与神经内分泌标志物的免疫反应性。
2. **标题**: "Functional Characterization of Secretogranin II-Derived Peptides in Hormone Secretion"
**作者**: Tanaka K, et al.
**摘要**: 通过重组SCG2蛋白的酶切实验，鉴定了其衍生肽段在调节胰岛素和生长激素分泌中的功能，揭示了其在代谢疾病中的潜在作用机制。
3. **标题**: "Secretogranin II as a Biomarker: Development of a Recombinant Protein-Based ELISA Assay"
**作者**: Müller S, et al.
**摘要**: 研究利用哺乳动物细胞表达的重组SCG2蛋白开发了高灵敏ELISA检测方法，成功应用于神经内分泌肿瘤患者的血清样本分析。
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**说明**：以上为模拟生成的参考文献，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"Secretogranin II recombinant" "SCG2 protein expression")。真实研究可能涵盖SCG2的病理生理功能、诊断应用或结构解析等内容。
Secretogranin II (SCG2), also known as Chromogranin C, is a member of the granin family of acidic secretory proteins predominantly expressed in neuroendocrine and neuronal cells. It is encoded by the SCG2 gene and functions as a prohormone precursor, processing into biologically active peptides such as secretoneurin and EM66 through proteolytic cleavage. These peptides participate in diverse physiological processes, including neurotransmission, hormone secretion, and vascular regulation. SCG2 is stored within dense-core secretory vesicles, where it plays a structural role in granule formation and cargo sorting alongside chromogranins A and B.
Recombinant SCG2 protein is engineered using expression systems like bacterial, yeast, or mammalian cells to produce purified, functional forms of the protein or its derived peptides. This technology enables researchers to study SCG2's molecular interactions, receptor binding mechanisms (e.g., secretoneurin's affinity for neuropeptide Y receptors), and its involvement in pathophysiological conditions. Elevated SCG2 levels have been observed in neuroendocrine tumors, cardiovascular diseases, and neurodegenerative disorders, positioning it as a potential diagnostic biomarker.
Current research focuses on leveraging recombinant SCG2 to explore its therapeutic potential in modulating inflammatory responses, neuronal survival, and endocrine functions. Its role in regulating catecholamine release and angiogenesis further highlights relevance to hypertension and cancer biology. As a tool compound, recombinant SCG2 facilitates mechanistic studies bridging neuroendocrinology and systemic physiology.
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