| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABI3 |
| Uniprot No | Q9P2A4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-366aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAELQQLQEFEIPTGREALRGNHSALL RVADYCEDNYVQATDKRKALEETMAFTTQALASVAYQVGNLAGHTLRMLD LQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPPGQKVIAP ENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATP ASATLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPK GQAAPPAPPLPSSLDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDL PPPPPLDGDELGLPPPPPGFGPDEPSWVPASYLEKVVTLYPYTSQKDNEL SFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGNYVEPSC |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABI3重组蛋白的3篇代表性文献示例(内容基于学术研究主题概括，具体文献需根据实际检索调整)：
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1. **文献名称**: *"Functional characterization of recombinant ABI3 protein in neuronal apoptosis regulation"*
**作者**: Smith J, et al. (2018)
**摘要**: 本研究通过大肠杆菌表达系统成功纯化重组ABI3蛋白，并发现其在体外神经元模型中显著抑制淀粉样蛋白诱导的细胞凋亡。实验表明ABI3可能通过与caspase-3相互作用调控凋亡通路。
2. **文献名称**: *"ABI3 recombinant protein suppresses tumor growth via Wnt/β-catenin signaling in lung adenocarcinoma"*
**作者**: Zhang L, et al. (2020)
**摘要**: 作者利用哺乳动物细胞表达体系制备重组ABI3蛋白，证实其通过抑制Wnt/β-catenin通路降低肺癌细胞增殖和转移能力，动物实验显示其显著延缓异种移植瘤生长。
3. **文献名称**: *"Structural insights into ABI3-DNA binding activity using recombinant protein mutagenesis"*
**作者**: Lee S, et al. (2019)
**摘要**: 本研究解析了重组ABI3蛋白的晶体结构，并通过点突变实验发现其N端结构域对DNA结合至关重要，为理解ABI3在基因转录调控中的作用机制提供结构基础。
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**注**：若需真实文献，建议在PubMed/Google Scholar中以 **"ABI3 recombinant protein"** 或 **"ABI3 overexpression"** 为关键词检索，筛选涉及重组蛋白表达、功能或机制研究的论文。部分相关研究可能涉及ABI3在癌症、神经疾病或免疫调控中的功能。
**Background of ABI3 Recombinant Protein**
ABI3 (ABI Family Member 3) is a protein encoded by the *ABI3* gene, initially identified for its role in the Abl interactor (ABI) protein family, which regulates cytoskeletal dynamics, cell signaling, and intracellular trafficking. ABI3 shares structural homology with ABI1 and ABI2. containing conserved domains such as a homeobox-like motif and SH3-binding regions, enabling interactions with proteins like Abl tyrosine kinases and components of the WAVE regulatory complex. These interactions position ABI3 as a modulator of actin polymerization, cell motility, and membrane remodeling.
Recombinant ABI3 protein is engineered through heterologous expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical and functional properties. Its production typically involves cloning the *ABI3* cDNA into expression vectors, followed by purification via affinity tags (e.g., His-tag). Recombinant ABI3 enables researchers to dissect its role in signaling pathways, particularly in immune regulation and neurodegenerative diseases. Notably, ABI3 has been implicated in Alzheimer’s disease pathogenesis, where its dysregulation may influence amyloid-beta processing and neuroinflammation.
Interest in ABI3 also extends to cancer biology, as aberrant expression correlates with tumor progression and metastasis. Recombinant forms facilitate structural studies, antibody development, and high-throughput drug screening. Despite progress, functional redundancy within the ABI family and context-dependent roles of ABI3 in different tissues remain under investigation. Advances in recombinant protein technology continue to enhance mechanistic insights, positioning ABI3 as a potential therapeutic target or biomarker in disease.
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