| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phase response factor |
| Entrez GeneID | 6774 |
| WB Predicted band size | Calculated MW: 88 kDa; Observed MW: 88 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT3. AA range:694-743 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是3篇关于STAT3抗体的经典文献摘要概括：
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1. **文献名称**: "STAT3 Activation via Interleukin-6 Family Ligands"
**作者**: Turkson, J. et al.
**摘要**: 研究开发了特异性识别磷酸化STAT3(Tyr705)的抗体，用于检测IL-6家族细胞因子诱导的STAT3激活机制，证实其在肿瘤细胞中的持续活化与增殖相关。
2. **文献名称**: "Constitutive Activation of STAT3 in Human Tumors"
**作者**: Yu, H. et al.
**摘要**: 利用STAT3特异性抗体进行免疫组化和Western blot分析，揭示多种实体瘤中STAT3的组成型激活现象，提出靶向STAT3作为潜在癌症治疗策略。
3. **文献名称**: "STAT3 Mutations in Human Disease"
**作者**: O'Shea, J.J. et al.
**摘要**: 通过STAT3抗体检测突变体蛋白的亚细胞定位及功能，发现STAT3基因突变导致免疫失调疾病(如高IgE综合征)，并影响Th17细胞分化。
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这些文献涵盖了STAT3抗体的开发、在疾病机制研究中的应用及治疗潜力。如需具体年份或期刊信息可进一步补充。
**Background of STAT3 Antibody**
The Signal Transducer and Activator of Transcription 3 (STAT3) is a transcription factor central to cellular processes such as proliferation, differentiation, apoptosis, and immune regulation. It is activated via phosphorylation (tyrosine residue Y705 or serine S727) by upstream kinases, including JAKs, receptor tyrosine kinases, or non-receptor kinases, in response to cytokines, growth factors, or oncogenic signals. Once activated, STAT3 dimerizes, translocates to the nucleus, and regulates the expression of target genes involved in inflammation, cancer progression, and immune evasion. Dysregulated STAT3 signaling is implicated in numerous pathologies, including cancers, autoimmune diseases, and chronic inflammatory conditions.
STAT3 antibodies are essential tools for studying its expression, activation, and functional roles. These antibodies are designed to detect specific STAT3 isoforms, phosphorylation states, or interaction partners via techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry, or chromatin immunoprecipitation (ChIP). Phospho-specific STAT3 antibodies (e.g., anti-pY705-STAT3) are particularly valuable for assessing activation status in disease models or therapeutic contexts.
Research leveraging STAT3 antibodies has advanced the development of targeted therapies, such as small-molecule inhibitors or monoclonal antibodies, aimed at blocking aberrant STAT3 signaling in cancers and inflammatory disorders. Additionally, these antibodies aid in biomarker discovery, mechanistic studies, and preclinical validation of STAT3-targeting drugs, underscoring their significance in both basic and translational research.
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