| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ESAM |
| Uniprot No | Q96AP7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-247aa |
| 氨基酸序列 | QLQLHLPANRLQAVEGGEVVLPAWYTLHGEVSSSQPWEVPFVMWFFKQKE KEDQVLSYINGVTTSKPGVSLVYSMPSRNLSLRLEGLQEKDSGPYSCSVN VQDKQGKSRGHSIKTLELNVLVPPAPPSCRLQGVPHVGANVTLSCQSPRS KPAVQYQWDRQLPSFQTFFAPALDVIRGSLSLTNLSSSMAGVYVCKAHNE VGTAQCNVTLEVSTGPGAVDHHHHHH |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ESAM重组蛋白的3篇代表性文献摘要：
1. **《Structural basis of ESAM-mediated cell-cell adhesion》**
*作者：Sakurai et al.*
摘要：通过解析ESAM重组蛋白的晶体结构，揭示了其胞外免疫球蛋白样结构域的二聚化机制，阐明了ESAM介导内皮细胞间同源黏附的分子基础。
2. **《ESAM regulates neutrophil transmigration under inflammatory conditions》**
*作者：Wegmann et al.*
摘要：研究利用重组ESAM蛋白阻断实验，证明ESAM通过调控血管内皮钙黏蛋白的分布，影响中性粒细胞在炎症部位的跨内皮迁移过程。
3. **《Recombinant ESAM-Fc modulates angiogenesis in tumor models》**
*作者：Ishida et al.*
摘要：开发ESAM-Fc重组融合蛋白，证实其通过竞争性抑制VEGF信号通路，显著抑制小鼠肿瘤模型中的病理性血管新生，提示其潜在抗肿瘤应用价值。
注：以上文献信息为学术场景模拟，实际引用请通过PubMed/Web of Science等数据库核实。建议结合研究主题补充"ESAM重组蛋白"在血栓形成、免疫调节等领域的相关文献。
ESAM (Endothelial Cell-Specific Adhesion Molecule) is a type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily, primarily expressed on vascular endothelial cells. It was first identified in the early 2000s as a key regulator of endothelial cell adhesion and vascular permeability. Structurally, ESAM consists of an extracellular region with two Ig-like domains, a single transmembrane domain, and a cytoplasmic tail containing conserved signaling motifs. Its homophilic interaction (ESAM-ESAM binding) plays a critical role in maintaining endothelial barrier integrity and modulating leukocyte transmigration during inflammation.
Research has linked ESAM to angiogenesis, thrombosis, and immune responses. It is notably upregulated during endothelial activation by inflammatory cytokines like TNF-α. In pathological conditions, ESAM participates in tumor angiogenesis by promoting endothelial cell proliferation and vascular remodeling. Additionally, its cytoplasmic domain interacts with signaling adaptors such as MAGI-1. influencing cell polarity and junctional stability.
Recombinant ESAM proteins, produced through bacterial or mammalian expression systems, are widely used to study these mechanisms. They enable in vitro analysis of ligand-receptor interactions, endothelial barrier function, and leukocyte adhesion assays. Therapeutic applications are being explored, including ESAM-targeted antibodies for inhibiting pathological angiogenesis in cancers or dampening excessive inflammation. Recent studies also suggest its potential as a biomarker for endothelial dysfunction in cardiovascular diseases. Despite progress, the full scope of ESAM's biological roles and clinical relevance remains an active area of investigation.
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