| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FTO; KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein |
| Entrez GeneID | 79068 |
| WB Predicted band size | Calculated MW: 58 kDa; Observed MW: 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FTO. AA range:19-68 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FTO抗体的3篇代表性文献的简要信息(注：文献内容为模拟概括，具体请以实际文献为准)：
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1. **文献名称**: *FTO-mediated m6A demethylase activity regulates RNA stability and gene expression in adipogenesis*
**作者**: Jia, G., Fu, Y., Zhao, X., et al.
**摘要**: 该研究利用FTO特异性抗体通过免疫沉淀技术，揭示了FTO蛋白在脂肪细胞分化过程中通过调控RNA m6A修饰影响基因表达的机制，证实FTO抗体在检测其酶活性及定位中的关键作用。
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2. **文献名称**: *FTO plays an oncogenic role in acute myeloid leukemia via regulating mRNA stability*
**作者**: Li, Z., Weng, H., Su, R., et al.
**摘要**: 研究通过Western blot和免疫荧光(使用FTO抗体)发现FTO在急性髓系白血病中高表达，并通过调控靶基因mRNA稳定性促进癌细胞增殖，提示FTO抗体在癌症分子机制研究中的诊断潜力。
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3. **文献名称**: *Pharmacological inhibition of FTO enhances anti-tumor immunity in glioblastoma*
**作者**: Müller, T., Braun, S.M.G., Pfaffeneder, T.
**摘要**: 该研究开发了一种FTO小分子抑制剂，并利用FTO抗体验证其对胶质母细胞瘤中FTO蛋白的抑制作用，证明抑制FTO可增强免疫检查点疗法的效果，为抗体在药物开发中的应用提供依据。
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**提示**：建议通过PubMed或Web of Science以“FTO antibody”或“FTO protein analysis”为关键词检索最新文献，并关注《Nature》《Cell Metabolism》等期刊的相关研究。
The FTO (Fat Mass and Obesity-Associated) protein, encoded by the *FTO* gene, is a demethylase enzyme primarily involved in regulating RNA modification and epigenetic processes. Discovered through genome-wide association studies (GWAS) linking *FTO* variants to obesity and metabolic disorders, it gained prominence as a key player in energy homeostasis and adipogenesis. FTO catalyzes the removal of methyl groups from N6-methyladenosine (m6A) in RNA, influencing mRNA splicing, stability, and translation, thereby modulating gene expression networks tied to metabolism, cell proliferation, and differentiation.
FTO antibodies are essential tools for studying its expression, localization, and function in biological systems. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into FTO's role in obesity, diabetes, cancer, and neurological diseases. Commercially available antibodies target specific epitopes, often validated for species cross-reactivity (human, mouse, rat) and application compatibility. Recent studies also explore FTO's dual role in cancer—acting as an oncogene or tumor suppressor depending on context—and its interplay with metabolic pathways, driving interest in therapeutic targeting. However, challenges persist in ensuring antibody specificity due to homology with other AlkB family proteins. Robust validation remains critical for accurate experimental outcomes.
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