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Rabbit Polyclonal Ubiquitin Antibody

  • 中文名： Ubiquitin抗体
  • 别    名： FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
货号: IPDX23347
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesFLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Entrez GeneID7314/7316/6233/7311
WB Predicted band sizeRefer to figures
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenThe antiserum was produced against synthesized peptide derived from human Ubiquitin. AA range:40-89
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是3篇关于泛素抗体的经典文献，供参考：

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1. **"Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulation"**

*作者：Kim HC, et al. (Nature, 2004)*

摘要：该研究报道了一种特异性识别K48连接型泛素链的单克隆抗体的开发，通过体外泛素链重构验证其结合特异性，并应用于检测细胞内蛋白酶体途径相关的多聚泛素化蛋白。

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2. **"Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies"**

*作者：Matsumoto ML, et al. (Cell, 2010)*

摘要：研究团队开发了针对K6、K11、K27等非经典泛素链连接类型的抗体，通过质谱和免疫印迹验证其特异性，揭示了泛素链编辑酶在DNA损伤修复中的作用。

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3. **"Phosphorylation of ubiquitin at Ser65 regulates its polymerization"**

*作者：Newton K, et al. (EMBO J, 2008)*

摘要：首次报道了针对泛素丝氨酸65磷酸化修饰(pS65-Ub)的抗体，该抗体用于检测帕金森病相关激酶PINK1调控的线粒体自噬通路中的泛素磷酸化事件。

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4. **"Methods to analyze ubiquitin conjugation and degradation"**

*作者：Yau RG, et al. (Methods Enzymol, 2019)*

摘要：综述类文献，系统总结了泛素抗体的选择策略(包括抗K63、M1连接型抗体)，以及它们在免疫沉淀、免疫荧光等技术中检测蛋白质泛素化的实验方法优化。

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这些文献覆盖了泛素抗体的开发、特异性验证及在关键生物学机制研究中的应用。如需具体实验细节或更近期研究，可进一步补充说明。

背景信息

Ubiquitin antibodies are essential tools for studying the ubiquitin-proteasome system (UPS), a critical pathway regulating protein degradation, signaling, and homeostasis in eukaryotic cells. Ubiquitin, a highly conserved 76-amino-acid protein, covalently attaches to substrate proteins via E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. This post-translational modification, called ubiquitination, can mark proteins for proteasomal degradation (via K48-linked polyubiquitin chains) or modulate non-degradative processes like DNA repair and inflammation (e.g., K63-linked chains). Ubiquitin antibodies detect specific forms of ubiquitin, including mono-ubiquitin, polyubiquitin chains with distinct linkage types (K48. K63. etc.), or phosphorylated ubiquitin variants. They are widely used in techniques like Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry to study UPS dynamics, protein turnover, and disease mechanisms. For example, antibodies targeting K48-linked chains help identify proteins destined for degradation, while those recognizing K63 linkages are used in studying NF-κB signaling or autophagy. Specificity is crucial, as cross-reactivity with unrelated ubiquitin-like proteins (e.g., SUMO, NEDD8) must be minimized. Dysregulation of ubiquitination is linked to cancers, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and immune disorders, making ubiquitin antibodies vital for both basic research and drug discovery targeting UPS components.

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