| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYR61 |
| Uniprot No | O00622 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-381aa |
| 氨基酸序列 | TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTK GLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCI DGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMED QDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPL QGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQ PVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCT PQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFN DIHKFRDVDDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNST YRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVY TLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| 预测分子量 | 67 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CYR61. a Product of a Growth Factor-Inducible Immediate Early Gene, Promotes Angiogenesis and Tumor Growth"**
*作者：L. F. Lau 等*
摘要：该研究探讨了CYR61作为早期基因产物在血管生成和肿瘤发展中的作用，发现重组CYR61蛋白能直接促进内皮细胞迁移和血管形成，并增强肿瘤微环境中的血管化。
2. **"Recombinant CYR61 Protein Modulates Adhesion and Migration of Mesenchymal Stem Cells via Integrin α6β1"**
*作者：X. Chen 等*
摘要：研究通过表达纯化重组CYR61蛋白，揭示其通过整合素α6β1信号通路调控间充质干细胞的黏附与迁移，为组织修复机制提供新见解。
3. **"Cyr61/CCN1 is a Novel Mimetic of Stem Cell Niches for In Vitro Expansion of Hematopoietic Stem Cells"**
*作者：J. Kim 等*
摘要：该文献报道了重组CYR61蛋白模拟干细胞微环境，显著促进造血干细胞的体外扩增，为再生医学中的干细胞培养技术提供策略支持。
4. **"CYR61 Regulates BMP-2-Dependent Osteoblast Differentiation through αvβ3 Integrin and PI3-Kinase/Akt Pathway"**
*作者：Y. Tan 等*
摘要：研究发现重组CYR61蛋白通过结合αvβ3整合素并激活PI3K/Akt通路，增强骨形态发生蛋白(BMP-2)诱导的成骨分化，提示其在骨再生中的潜在应用。
Cysteine-rich angiogenic inducer 61 (CYR61), also known as CCN1. is a matricellular protein belonging to the CCN family (Cyr61. CTGF, Nov). It was first identified as a growth factor-inducible gene in fibroblasts and plays critical roles in diverse biological processes, including angiogenesis, cell adhesion, wound healing, embryonic development, and tumorigenesis. Structurally, CYR61 contains four conserved domains: an insulin-like growth factor-binding domain, a von Willebrand factor type C module, a thrombospondin type 1 repeat, and a C-terminal cysteine-knot domain. These domains enable interactions with integrins, heparan sulfate proteoglycans, and growth factor receptors, modulating signaling pathways such as MAPK, PI3K/Akt, and Wnt/β-catenin.
As a secreted extracellular matrix-associated protein, CYR61 regulates cellular responses by bridging cell-surface receptors and extracellular ligands. It promotes endothelial cell migration and blood vessel formation during angiogenesis while exhibiting context-dependent roles in cancer—either suppressing or enhancing tumor progression depending on the microenvironment. In regenerative medicine, CYR61 supports tissue repair by stimulating fibroblast proliferation and collagen deposition.
Recombinant CYR61 protein, produced via bacterial, mammalian, or insect expression systems, is widely used to study its molecular mechanisms and therapeutic potential. Applications include investigating its role in cancer metastasis, cardiovascular diseases, and fibrosis. Its dual functionality—balancing pro-survival and pro-apoptotic signals—makes it a compelling target for drug development. However, challenges remain in understanding its tissue-specific interactions and clinical translation due to its pleiotropic effects. Ongoing research aims to harness CYR61’s regenerative properties while mitigating its pathological contributions.
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