| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CFC1 |
| Uniprot No | P0CG37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-158aa |
| 氨基酸序列 | YQREK HNGGREEVTK VATQKHRQSP LNWTSSHFGE VTGSAEGWGP EEPLPYSRAF GEGASARPRC CRNGGTCVLG SFCVCPAHFT GRYCEHDQRR SECGALEHGA WTLRACHLCR CIFGALHCLP LQTPDRCD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CFC1重组蛋白的3篇代表性文献的示例性概述(注：文献为模拟示例，实际引用需核实)：
1. **《Functional characterization of recombinant CFC1 protein in Nodal signaling》**
*作者：Shen, M.M. et al.*
摘要：研究通过昆虫细胞系统表达重组CFC1蛋白，证实其作为Nodal信号通路的共受体，通过体外结合实验证明CFC1与ALK4/ActRIIB受体复合物相互作用，增强信号转导效率。
2. **《Crystal structure of human CFC1 domain reveals its role in embryonic left-right patterning》**
*作者：Branford, W.W. et al.*
摘要：利用大肠杆菌表达并纯化人源CFC1的EGF样结构域重组蛋白，通过X射线晶体学解析其三维结构，揭示了关键氨基酸残基如何参与配体识别，为先天性心脏病机制提供结构依据。
3. **《Recombinant CFC1 rescues cardiac defects in zebrafish cfc1 mutants》**
*作者：Yan, Y.T. et al.*
摘要：在斑马鱼模型中注射哺乳动物细胞表达的重组CFC1蛋白，成功修复胚胎心脏左右不对称发育异常，证明外源性CFC1蛋白可补偿基因突变导致的发育缺陷。
CFC1 (Cripto, FRL-1. Cryptic family protein 1) is a key developmental protein involved in embryonic patterning and cell differentiation. As a member of the EGF-CFC family, it acts as an essential co-receptor for Nodal signaling, a TGF-β signaling pathway critical for establishing left-right asymmetry, germ layer formation, and organogenesis during early embryogenesis. The CFC1 protein contains a conserved EGF-like domain and a CFC-specific motif that facilitates interactions with Nodal ligands and their receptors.
Recombinant CFC1 protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, biologically active forms for research applications. Its production enables studies on molecular mechanisms of embryonic development, particularly in modeling congenital disorders like heterotaxy syndrome and congenital heart defects linked to CFC1 mutations. Researchers also utilize recombinant CFC1 to investigate its role in stem cell differentiation and tissue regeneration, given its influence on maintaining pluripotency and directing cell fate.
Structurally, the recombinant protein often retains post-translational modifications (e.g., glycosylation) when expressed in mammalian systems, mimicking native functionality. Current research explores its potential as a therapeutic target or biomarker for cancers, as aberrant Nodal/CFC1 signaling has been implicated in tumor progression. However, challenges remain in fully elucidating its context-dependent signaling dynamics and off-target effects in therapeutic applications.
Overall, CFC1 recombinant protein serves as a vital tool for developmental biology and translational research, bridging insights from embryogenesis to human disease mechanisms.
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