| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPDC1 |
| Uniprot No | Q9NQX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-181aa |
| 氨基酸序列 | GHPDVAACPGSLDCALKRRARCPPGAHACGPCLQPFQEDQQGLCVPRMRR PPGGGRPQPRLEDEIDFLAQELARKESGHSTPPLPKDRQRLPEPATLGFS ARGQGLELGLPSTPGTPTPTPHTSLGSPVSSDPVHMSPLEPRGGQGDVDH HHHHH |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and functional analysis of recombinant NPDC1 in neural stem cell differentiation"**
- **作者**: Zhang L, et al.
- **摘要**: 研究通过大肠杆菌表达系统成功制备了重组NPDC1蛋白，并发现其通过抑制PI3K/AKT通路调控神经干细胞的增殖与分化，为神经再生研究提供工具。
2. **"Purification and characterization of NPDC1 as a tumor suppressor in lung cancer models"**
- **作者**: Tanaka K, et al.
- **摘要**: 利用哺乳动物细胞系表达并纯化功能性NPDC1重组蛋白，证实其通过结合p53增强抑癌活性，抑制肺癌细胞迁移与侵袭。
3. **"NPDC1 recombinant protein attenuates hepatic fibrosis via TGF-β/Smad signaling inhibition"**
- **作者**: Wang Y, et al.
- **摘要**: 通过昆虫杆状病毒系统生产高纯度NPDC1重组蛋白，动物实验表明其通过阻断TGF-β/Smad通路显著减轻肝纤维化，提示潜在治疗应用。
4. **"Structural insights into NPDC1 interactions with cyclin-dependent kinases"**
- **作者**: Müller R, et al.
- **摘要**: 采用真核表达系统获得NPDC1重组蛋白，结合X射线晶体学解析其与CDK2的复合物结构，揭示了其调控细胞周期停滞的分子机制。
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注：上述文献为示例性内容，实际引用需根据具体研究检索PubMed、Web of Science等数据库获取。若需真实文献线索，可限定关键词(如"NPDC1 recombinant protein expression")筛选近年研究。
**Background of NPDC1 Recombinant Protein**
Neural Proliferation, Differentiation and Control-1 (NPDC1) is a nuclear protein encoded by the *NPDC1* gene, initially identified for its role in regulating neural cell proliferation and differentiation. It is highly conserved across species and shares structural similarities with transcriptional regulators, suggesting involvement in gene expression modulation. NPDC1 is predominantly expressed in the brain, retina, and testes, with emerging roles in cancer biology, neurodegenerative disorders, and metabolic regulation.
Structurally, NPDC1 contains a coiled-coil domain and nuclear localization signals, enabling interactions with proteins like E2F transcription factors and cyclin-dependent kinase inhibitors (e.g., p21). These interactions link NPDC1 to cell cycle control, apoptosis, and neuronal development. Studies highlight its tumor-suppressive potential in cancers (e.g., glioblastoma, breast cancer) by inhibiting proliferation and promoting differentiation. Conversely, dysregulated NPDC1 expression correlates with poor prognosis in certain malignancies, indicating context-dependent roles.
Recombinant NPDC1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce functional, soluble protein for research. It enables mechanistic studies of NPDC1’s interactions, post-translational modifications (e.g., phosphorylation), and downstream pathways (e.g., PI3K/Akt, MAPK). Applications include *in vitro* assays, antibody development, and drug screening. For instance, recombinant NPDC1 has been used to explore its neuroprotective effects in Alzheimer’s models and its metabolic roles in insulin signaling.
Overall, NPDC1 recombinant protein serves as a critical tool to unravel the protein’s multifaceted roles in health and disease, offering potential therapeutic avenues.
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