| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GRB2; ASH; Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2 |
| Entrez GeneID | 2885 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human GRB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRB2抗体的3篇参考文献示例(内容为模拟，非真实文献)：
1. **"GRB2 Antibody Validation in EGFR Signaling Pathways"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过免疫沉淀和Western blot验证了GRB2抗体在EGFR信号转导中的特异性，发现GRB2作为衔接蛋白介导Ras/MAPK通路激活，抗体成功检测到配体刺激后GRB2与EGFR的相互作用。
2. **"Role of GRB2 in Immune Receptor Signaling: Antibody-Based Functional Analysis"**
- **作者**: Lee C, et al.
- **摘要**: 利用特异性GRB2抗体研究其在T细胞受体信号中的作用，证实GRB2通过结合磷酸化酪氨酸残基调控下游PI3K/Akt通路，抗体在流式细胞术和免疫荧光中表现优异。
3. **"GRB2 Expression in Cancer: A Comparative Study Using Commercial Antibodies"**
- **作者**: Wang Y, et al.
- **摘要**: 对比多种市售GRB2抗体的灵敏度和特异性，发现克隆号X的抗体在乳腺癌组织免疫组化中效果最佳，高GRB2表达与患者预后不良相关。
4. **"Structural and Functional Characterization of GRB2 Antibodies for Therapeutic Targeting"**
- **作者**: Brown K, et al.
- **摘要**: 开发并验证了一种新型单克隆GRB2抗体，通过阻断SH2结构域抑制肿瘤细胞迁移，抗体在小鼠模型中显著降低转移性肺癌进展。
(注：以上为模拟文献，实际引用时需查询真实数据库如PubMed或Google Scholar获取具体信息。)
The GRB2 (Growth Factor Receptor-Bound Protein 2) antibody is a critical tool for studying the role of GRB2. an adapter protein involved in intracellular signal transduction. GRB2 contains one Src homology 2 (SH2) domain and two Src homology 3 (SH3) domains, enabling it to link activated receptor tyrosine kinases (RTKs) or other phosphorylated signaling molecules to downstream effectors like SOS1. which activates the RAS/MAPK pathway. This protein is essential for regulating cell proliferation, differentiation, and survival, and its dysregulation is implicated in cancer, immune disorders, and developmental defects.
GRB2 antibodies are widely used in research to detect GRB2 expression levels, localization, and protein-protein interactions. They are employed in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and flow cytometry. These antibodies help identify GRB2’s involvement in signaling pathways triggered by growth factors (e.g., EGF, insulin) or cytokines, as well as its role in pathological conditions like oncogenesis. For example, GRB2 overexpression has been linked to HER2-positive breast cancer and leukemia progression.
Researchers also use GRB2 antibodies to explore therapeutic targets, such as disrupting GRB2-mediated signaling in cancer treatment. Specificity validation (via knockout controls) and cross-reactivity checks are critical for experimental accuracy. Overall, GRB2 antibodies provide insights into cellular communication mechanisms and disease pathways, bridging basic research and clinical applications.
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