| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALML3 |
| Uniprot No | P27482 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMADQLTEEQVTEFKEAFSLFDKDGDG CITTRELGTVMRSLGQNPTEAELRDMMSEIDRDGNGTVDFPEFLGMMARK MKDTDNEEEIREAFRVFDKDGNGFVSAAELRHVMTRLGEKLSDEEVDEMI RAADTDGDGQVNYEEFVRVLVSK |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CALML3重组蛋白的假设性参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**: *"Expression and Purification of Recombinant CALML3 in Escherichia coli"*
**作者**: Zhang L, et al.
**摘要**: 本研究报道了CALML3基因在大肠杆菌系统中的重组表达与纯化，通过优化诱导条件提高蛋白可溶性，并利用亲和层析技术获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**: *"CALML3 Recombinant Protein Inhibits Tumor Cell Proliferation via Calcium Signaling Modulation"*
**作者**: Wang Y, et al.
**摘要**: 通过体外实验证明，重组CALML3蛋白可结合钙离子并调控下游信号通路，抑制多种癌细胞增殖，提示其在肿瘤治疗中的潜在应用价值。
3. **文献名称**: *"Structural Characterization of CALML3 Using X-ray Crystallography"*
**作者**: Tanaka K, et al.
**摘要**: 首次解析了重组CALML3蛋白的晶体结构，揭示了其与钙调蛋白家族其他成员的构象差异，为功能机制研究提供结构依据。
4. **文献名称**: *"CALML3 Recombinant Protein Interacts with MYO5A in Epithelial Cells"*
**作者**: Chen H, et al.
**摘要**: 利用重组CALML3蛋白进行Pull-down实验，发现其与MYO5A蛋白存在直接相互作用，可能参与细胞骨架动态调节过程。
**建议**：实际文献请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索关键词 "CALML3 recombinant protein" 或 "CALML3 expression" 获取最新研究。
**Background of CALML3 Recombinant Protein**
CALML3 (Calmodulin-like protein 3) is a member of the calmodulin protein family, which plays critical roles in calcium-mediated signaling pathways. As a calcium-binding protein, CALML3 shares structural homology with calmodulin (CaM), including EF-hand motifs that enable it to interact with calcium ions. However, CALML3 exhibits distinct tissue-specific expression patterns and functional specializations. It is predominantly expressed in epithelial tissues, such as the skin, and has been implicated in regulating cellular processes like differentiation, proliferation, and apoptosis.
The recombinant form of CALML3 is engineered using biotechnological methods, typically through cloning the CALML3 gene into expression vectors (e.g., bacterial or mammalian systems) to produce purified protein for research. Recombinant CALML3 retains the calcium-binding capacity and structural integrity of the native protein, enabling studies on its molecular interactions and mechanisms. Research highlights its role in maintaining epidermal homeostasis, where it modulates keratinocyte differentiation by interacting with proteins like transglutaminases or cytoskeletal components. Dysregulation of CALML3 has been linked to skin disorders, such as psoriasis, and certain cancers, suggesting its potential as a therapeutic target or biomarker.
Studies using recombinant CALML3 also explore its unique signaling properties compared to other calmodulin-like proteins, shedding light on tissue-specific calcium signaling networks. Its application extends to drug discovery, structural biology, and functional assays aimed at deciphering calcium-dependent regulatory mechanisms in health and disease.
×
