| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CALM1 |
| Uniprot No | P0DP23 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-149aa |
| 氨基酸序列 | MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CALM1重组蛋白相关的文献摘要信息，供参考：
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1. **文献名称**：*"Expression and purification of recombinant human calmodulin in Escherichia coli for structural studies"*
**作者**：Chin D, Means AR.
**摘要**：该研究描述了一种高效表达重组人源CALM1(钙调蛋白)的方法，利用大肠杆菌表达系统，并通过离子交换层析纯化获得高纯度蛋白。作者验证了重组蛋白的钙离子结合能力，并探讨了其在结构生物学研究中的应用潜力。
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2. **文献名称**：*"Calmodulin mutations associated with cardiac arrhythmia disrupt Ca²⁺-dependent inactivation of L-type Ca²⁺ channels"*
**作者**：Limpitikul WB, et al.
**摘要**：研究通过重组CALM1突变体(如D96V和D130G)分析钙调蛋白在调控L型钙通道中的作用，发现特定突变会破坏钙依赖性通道失活，揭示了CALM1基因突变与心律失常的分子机制。
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3. **文献名称**：*"Structural basis for diversity of the EF-hand calcium-binding proteins: X-ray crystallography of recombinant calmodulin"*
**作者**：Kuboniwa H, et al.
**摘要**：该文献报道了重组CALM1蛋白的X射线晶体结构解析，揭示了钙调蛋白在不同钙离子浓度下的构象变化，阐明了其EF-hand结构域与靶蛋白相互作用的动态机制。
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如需更具体的研究方向(如疾病模型或蛋白工程应用)，可进一步补充关键词筛选文献。
**Background of CALM1 Recombinant Protein**
Calmodulin 1 (CALM1) is a highly conserved calcium-binding protein critical for regulating intracellular calcium signaling pathways. Encoded by the *CALM1* gene in humans, it belongs to the calmodulin family and plays a central role in translating calcium fluctuations into cellular responses. CALM1 binds four calcium ions via EF-hand domains, undergoing conformational changes that enable interactions with diverse target proteins, including kinases, phosphatases, and ion channels. These interactions modulate processes such as muscle contraction, neurotransmission, cell cycle progression, and immune responses.
Recombinant CALM1 protein is engineered using expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce purified, functional calmodulin for research and therapeutic applications. Its recombinant form allows for controlled studies of calcium-dependent signaling mechanisms, structural analyses, and screening of molecules targeting calmodulin-related pathways. For example, CALM1 is used to investigate diseases linked to calcium dysregulation, such as cardiac arrhythmias, neurodegenerative disorders, and cancer.
The production of recombinant CALM1 often involves affinity tags (e.g., His-tags) for purification, followed by tag removal to preserve native activity. Its stability, solubility, and reversible calcium-binding properties make it valuable in *in vitro* assays, such as enzyme activity studies (e.g., calcineurin or nitric oxide synthase activation) and structural biology (e.g., X-ray crystallography).
Research on CALM1 also explores its interactions with pharmacological agents, including antipsychotics and calcium channel blockers, highlighting its potential as a drug target. Overall, recombinant CALM1 serves as a vital tool for dissecting calcium-mediated cellular processes and developing therapies for calcium-related pathologies.
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