| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DAC; CES5A1 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human AADAC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CKIIα抗体的3篇参考文献示例(内容为模拟生成，实际引用请核实原文)：
1. **文献名称**：*Casein Kinase IIα Antibody Validation in Cancer Signaling Studies*
**作者**：Miller, J. et al.
**摘要**：研究验证了商业来源的CKIIα抗体在Western blot和免疫组化中的特异性，发现其在多种癌细胞系中可特异性识别磷酸化及非磷酸化形式的CKIIα，并揭示了CKIIα过表达与肿瘤增殖的关系。
2. **文献名称**：*Structural Insights into CKIIα Kinase Domain via Monoclonal Antibody Staining*
**作者**：Chen, L. & Rodriguez, S.
**摘要**：报道了一种新型单克隆抗体的开发，该抗体通过靶向CKIIα的C端结构域实现高亲和力结合，成功用于共聚焦显微镜观察亚细胞定位，证实CKIIα在核内与DNA修复蛋白存在共定位。
3. **文献名称**：*CKIIα-Specific Antibody Reveals Dynamic Phosphorylation in Cell Cycle Regulation*
**作者**：Wang, X. et al.
**摘要**：利用CKIIα特异性抗体进行流式细胞术分析，发现其在G1/S期调控中通过磷酸化下游底物p21促进细胞周期进程，并证明抗体对磷酸化位点Ser362具有选择性识别能力。
(注：以上为虚构案例，实际文献请通过PubMed或Web of Science以"CKIIα antibody"或"casein kinase II alpha antibody"为关键词检索。)
**Background of CKII Alpha Antibody**
Casein Kinase II (CKII) is a ubiquitously expressed serine/threonine protein kinase involved in diverse cellular processes, including cell proliferation, apoptosis, DNA repair, and signal transduction. The CKII holoenzyme typically exists as a tetramer composed of two catalytic subunits (α and/or α') and two regulatory β subunits. The alpha subunit (CKIIα) is the primary catalytic component responsible for phosphorylating numerous substrates, ranging from transcription factors (e.g., p53. NF-κB) to metabolic enzymes.
CKIIα antibodies are essential tools for studying the expression, localization, and functional roles of CKIIα in both normal and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to detect CKIIα levels or assess its activity in various tissues and cell lines.
Research highlights CKIIα's involvement in cancer progression, as its overexpression or hyperactivation is linked to tumorigenesis, metastasis, and therapy resistance. CKIIα antibodies have thus become valuable in cancer research, aiding in biomarker discovery and therapeutic targeting. Additionally, they contribute to studies on neurodegenerative diseases, viral infections, and developmental disorders where CKII signaling is dysregulated.
Most CKIIα antibodies target conserved epitopes, ensuring cross-reactivity across species (e.g., human, mouse, rat). Validation via knockout controls or activity assays is critical to confirm specificity, given CKIIα's structural similarity to other kinases. Overall, CKIIα antibodies remain indispensable for unraveling the kinase's multifaceted roles in health and disease.
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