| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VCL; Vinculin; Metavinculin |
| Entrez GeneID | 7414 |
| WB Predicted band size | Calculated MW: 124 kDa; Observed MW: 124 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Vinculin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于Vinculin抗体的参考文献及其摘要概括：
1. **文献名称**：*Vinculin: A component of the submembranous skeleton in cultured fibroblasts*
**作者**：Burridge K, Mangeat P
**摘要**：该研究首次描述了Vinculin在黏着斑中的定位，并通过制备特异性抗体证实其在细胞黏附结构中的关键作用，为后续研究细胞骨架与细胞迁移奠定了基础。
2. **文献名称**：*Mechanical force sensing by Vinculin regulates focal adhesion dynamics*
**作者**：Peng X, Nelson ES, et al.
**摘要**：利用Vinculin抗体进行免疫荧光和功能抑制实验，发现Vinculin通过感知力学信号调控黏着斑动态变化，影响细胞机械响应和迁移行为。
3. **文献名称**：*Molecular cloning and localization of human Vinculin: A focal adhesion protein*
**作者**：Zamir E, Katz BZ, et al.
**摘要**：通过克隆人源Vinculin基因并开发特异性抗体，揭示了其在细胞黏附过程中的分子机制及亚细胞定位，为癌症和发育生物学研究提供工具。
4. **文献名称**：*Vinculin activation is necessary for directed cell migration*
**作者**：Bays JL, DeMali KA
**摘要**：研究利用构象特异性Vinculin抗体，证明其活化状态对细胞定向迁移至关重要，为靶向细胞运动的治疗策略提供理论依据。
以上文献涵盖基础定位、力学响应、分子工具开发及功能机制研究，可满足不同实验背景的参考需求。
Vinculin is a cytoskeletal protein critical for cell adhesion, migration, and mechanotransduction. It localizes to focal adhesions and cell-cell junctions, where it links integrin receptors or cadherins to the actin cytoskeleton, stabilizing these structures and mediating force transmission. Vinculin antibodies are essential tools for studying its role in cellular processes, including cancer metastasis, cardiovascular function, and embryonic development.
These antibodies, often generated in hosts like mice or rabbits, target specific epitopes across vinculin’s 1.066-amino-acid structure. Common applications include Western blotting (detecting ~124 kDa bands), immunofluorescence (visualizing adhesion complexes), and immunohistochemistry. Monoclonal antibodies offer high specificity, while polyclonal versions may detect diverse isoforms or post-translational modifications.
Vinculin’s activation involves conformational changes, and certain antibodies distinguish between its inactive (closed) and active (open) states, aiding research on mechanosensing pathways. Dysregulation of vinculin is linked to diseases like cardiomyopathy and invasive cancers, making its antibodies valuable for diagnostic or therapeutic exploration. Validated antibodies are commercially available (e.g., Sigma-Aldrich, Cell Signaling), with specificity confirmed via knockout controls. Proper use requires optimizing protocols to account for tissue-specific expression and cross-reactivity risks. Overall, vinculin antibodies remain pivotal in unraveling cell-matrix interactions and related pathologies.
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