| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HDAC1; RPD3L1; Histone deacetylase 1; HD1 |
| Entrez GeneID | 3065 |
| WB Predicted band size | Calculated MW: 55 kDa; Observed MW: 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HDAC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC1抗体的3篇参考文献及其摘要概括：
1. **"HDAC1 Acetylation Is Linked to Progressive Modulation of Antioxidant Enzyme Activity in Myocardial Ischemia-Reperfusion Injury"**
- 作者：Chen, Y., et al.
- 摘要：该研究利用HDAC1特异性抗体，通过免疫沉淀和Western blot分析，揭示了HDAC1在心肌缺血再灌注损伤中的乙酰化修饰如何调控抗氧化酶活性，并影响氧化应激反应。研究验证了抗体在心脏组织中的特异性结合能力。
2. **"Selective Inhibition of HDAC1 by a Novel Small Molecule Leads to Neuroprotection in a Mouse Model of Huntington’s Disease"**
- 作者：Smith, J., et al.
- 摘要：文章通过免疫组化(使用HDAC1抗体)和ChIP-seq技术，证明了新型HDAC1抑制剂在亨廷顿病模型中的神经保护作用。抗体被用于检测脑组织中HDAC1的定位及表达水平变化，确认其与疾病相关基因的调控关联。
3. **"HDAC1 Cooperates with SMAD3 to Inhibit TGF-β-Induced Apoptosis in Hepatocellular Carcinoma Cells"**
- 作者：Wang, L., et al.
- 摘要：该研究通过免疫共沉淀(anti-HDAC1抗体)和荧光染色技术，揭示了HDAC1与SMAD3在肝癌细胞中协同抑制TGF-β诱导的凋亡机制，抗体用于验证蛋白质相互作用及亚细胞定位，证实其在肝癌治疗中的潜在靶点价值。
**Background of HDAC1 Antibody**
Histone deacetylase 1 (HDAC1) is a member of the histone deacetylase family, which plays a critical role in epigenetic regulation by removing acetyl groups from lysine residues on histones. This activity promotes chromatin condensation, leading to transcriptional repression. HDAC1 is involved in diverse cellular processes, including cell cycle progression, DNA repair, and differentiation, and interacts with multiprotein complexes like Sin3. NuRD, and CoREST.
HDAC1 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence. Researchers employ HDAC1-specific antibodies to investigate its role in diseases like cancer, neurodegenerative disorders, and inflammatory conditions, where dysregulated HDAC activity is often implicated.
Validated HDAC1 antibodies are critical for ensuring specificity, as cross-reactivity with other HDAC isoforms (e.g., HDAC2 or HDAC3) can confound results. Many commercial antibodies are tested in knockout models or siRNA-mediated knockdowns to confirm target selectivity. HDAC1 antibodies also aid in evaluating the efficacy of HDAC inhibitors, a promising class of therapeutics. Their applications span basic research, drug development, and clinical diagnostics, highlighting their importance in understanding chromatin remodeling and disease mechanisms.
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