| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPB1 |
| Uniprot No | P15086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-417aa |
| 氨基酸序列 | HHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHS TVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHS YEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQN KPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVL PVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASR NPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYS YAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDW AYDQGIRYSF TFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLYVDHHHHHH |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于CPB1(Carboxypeptidase B1)重组蛋白的参考文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**: *Expression and Purification of Recombinant Human CPB1 in Insect Cells*
**作者**: Smith A, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞表达系统成功表达并纯化了重组人源CPB1蛋白，优化了表达条件，并通过质谱和酶活实验验证了其功能活性，为后续酶学研究和药物开发提供了材料基础。
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2. **文献名称**: *Structural Insights into CPB1 Substrate Specificity by X-ray Crystallography*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组CPB1蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了CPB1选择性切割C端碱性氨基酸的分子机制。
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3. **文献名称**: *Role of CPB1 in Pancreatic Cancer Progression: In Vitro and In Vivo Studies*
**作者**: Wang Y, et al.
**摘要**: 利用重组CPB1蛋白进行功能实验，发现其在胰腺癌细胞侵袭转移中通过调控细胞外基质重塑发挥作用，动物模型显示抑制CPB1可减缓肿瘤生长。
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4. **文献名称**: *Development of a High-Throughput Assay for CPB1 Inhibitor Screening*
**作者**: Gonzalez R, et al.
**摘要**: 基于重组CPB1蛋白建立了荧光底物高通量筛选平台，筛选出多个小分子抑制剂，并验证了其对CPB1酶活的抑制作用，为治疗炎症性疾病提供候选化合物。
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(注：以上文献为示例性内容，实际研究中需通过PubMed、Web of Science等数据库检索真实文献。)
**Background of CPB1 Recombinant Protein**
Carboxypeptidase B1 (CPB1), also known as carboxypeptidase B, is a zinc-dependent metalloprotease that plays a critical role in the selective cleavage of basic amino acids (lysine or arginine) from the C-terminus of proteins and peptides. This enzyme is part of the peptidase M14 family and is naturally produced in the pancreas as a zymogen (pro-CPB1), which is activated by trypsin-mediated cleavage. Its primary physiological function involves regulating peptide hormone maturation, fibrinogen processing, and extracellular matrix remodeling.
Recombinant CPB1 protein is engineered using genetic recombination technology, typically expressed in host systems like *E. coli*, yeast, or mammalian cells*,* to ensure high purity and activity for research or industrial applications. The recombinant form retains the enzymatic properties of native CPB1. including substrate specificity and optimal activity at neutral to slightly alkaline pH. Structurally, it features a conserved catalytic domain with a zinc-binding motif critical for its hydrolytic function.
In biotechnology, recombinant CPB1 is widely utilized in insulin production, where it removes C-terminal arginine or lysine residues from pro-insulin to generate mature, biologically active insulin. It also serves as a tool in proteomics for peptide mapping and protein sequencing. Additionally, CPB1 has therapeutic relevance, with studies exploring its role in inflammatory diseases, cancer metastasis, and tissue repair due to its ability to modulate bioactive peptides.
Despite its utility, differences in post-translational modifications between recombinant and native CPB1 may influence activity in certain contexts, requiring careful characterization for specific applications. Ongoing research continues to refine production methods and expand its applications in both industrial and biomedical fields.
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