| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SEMA3C |
| Uniprot No | Q99985 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-738aa |
| 氨基酸序列 | GSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGS KDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQ TFNRTHLYVCGSGAFSPVCTYLNRGRRSEDQVFMIDSKCESGKGRCSFNP NVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRTDQHNSKWLSE PMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDT GGLRSLVNKWTTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTL VYGIFTTSSSVFKGSAVCVYHLSDIQTVFNGPFAHKEGPNHQLISYQGRI PYPRPGTCPGGAFTPNMRTTKEFPDDVVTFIRNHPLMYNSIYPIHKRPLI VRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNNSVSG ELILEELEVFKNHAPITTMKISSKKQQLYVSSNEGVSQVSLHRCHIYGTA CADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNPLTQCRGFNLK AYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNER IIATSQGLLIRSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVV TDKWSPWTWASSVRALPFHPKDIMGAFSHSEMQMINQYCKDTRQQHQQGD ESQKMRGDYGKLKALINSVDHHHHHH |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SEMA3C重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*SEMA3C promotes cancer progression by binding to integrin β1 and activating the AKT/ERK signaling pathway*
**作者**：Zhang Y, et al.
**摘要**：研究发现重组SEMA3C蛋白通过结合整合素β1激活AKT/ERK信号通路，促进肿瘤细胞侵袭和转移，揭示了其在癌症进展中的关键作用。
2. **文献名称**：*Structural insights into semaphorin-3C signaling through plexin-B1*
**作者**：Janssen B, et al.
**摘要**：该研究解析了重组SEMA3C蛋白与其受体plexin-B1的复合物结构，阐明了其介导细胞迁移抑制的分子机制，为靶向干预提供了结构基础。
3. **文献名称**：*SEMA3C drives resistance to targeted therapy in EGFR-mutant lung cancer*
**作者**：Wang H, et al.
**摘要**：利用重组SEMA3C蛋白验证其通过激活MAPK通路介导EGFR突变肺癌的靶向治疗耐药，提示其作为潜在治疗靶点。
4. **文献名称**：*Semaphorin 3C regulates embryonic heart development via neuropilin-1*
**作者**：Li Y, et al.
**摘要**：通过重组蛋白功能实验，发现SEMA3C通过神经纤毛蛋白-1(NRP1)调控胚胎心脏的血管形成和心肌细胞迁移，影响心脏发育。
(注：以上文献信息为简化示例，实际引用需核对具体论文细节。)
**Background of SEMA3C Recombinant Protein**
SEMA3C, a member of the class 3 semaphorin family, is a secreted glycoprotein known for its dual role in regulating cellular processes such as axon guidance, angiogenesis, cell migration, and immune modulation. Initially identified for its function in neural development, SEMA3C binds to receptor complexes comprising neuropilins (NRP1/2) and plexins, triggering downstream signaling pathways (e.g., MAPK, PI3K/Akt) that influence cytoskeletal dynamics and cell-cell communication. Structurally, it contains a conserved N-terminal Sema domain, followed by immunoglobulin-like (Ig) and thrombospondin-like (TSP) motifs, critical for receptor interaction and functional specificity.
In disease contexts, SEMA3C exhibits paradoxical roles. While it acts as a tumor suppressor in certain cancers by inhibiting cell proliferation and metastasis, it also promotes tumorigenesis in others by enhancing angiogenesis, epithelial-mesenchymal transition (EMT), and drug resistance. Dysregulation of SEMA3C is linked to pathologies such as glioblastoma, prostate cancer, and cardiovascular disorders.
Recombinant SEMA3C protein, produced via mammalian or insect expression systems, retains bioactive properties and is widely used to study its molecular mechanisms. Purified through affinity chromatography, it serves as a tool for *in vitro* and *in vivo* assays to dissect its role in development and disease. Recent research explores its therapeutic potential, including targeting SEMA3C-NRP/plexin axis in cancer or leveraging its anti-angiogenic effects in ocular diseases. However, challenges remain in understanding context-dependent signaling and optimizing delivery strategies for clinical applications.
Overall, SEMA3C recombinant protein remains a critical reagent for unraveling the complexity of semaphorin biology and advancing translational studies.
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