| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2 |
| Entrez GeneID | 3717 |
| WB Predicted band size | Calculated MW: 131 kDa; Observed MW: 131 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human JAK2 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于JAK2抗体的参考文献，涵盖其在疾病诊断及分子机制中的研究：
1. **"Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders"**
- **作者**: Baxter, EJ 等 (2005)
- **摘要**: 该研究首次在《Lancet》报道了JAK2 V617F突变与骨髓增殖性疾病(如真性红细胞增多症)的关联，利用特异性抗体通过功能实验证实突变导致JAK2激酶活性异常激活，为后续抗体检测技术的开发奠定基础。
2. **"Comparison of JAK2 V617F mutation detection assays in myeloproliferative neoplasms"**
- **作者**: Mead, AJ 等 (2012)
- **摘要**: 发表于《British Journal of Haematology》，系统比较了包括等位基因特异性PCR、焦磷酸测序及基于抗体的免疫分析法(如ELISA)在内的多种JAK2突变检测方法，指出抗体法在临床筛查中具有快速、低成本的优势。
3. **"The role of JAK2 inhibitors and JAK2 antibody-based diagnostics in hematologic malignancies"**
- **作者**: Tefferi, A 等 (2013)
- **摘要**: 这篇综述于《Blood》探讨了JAK2靶向治疗药物及抗体在血液肿瘤中的应用，强调抗JAK2单克隆抗体在组织活检免疫组化(IHC)中的高特异性，可辅助鉴别JAK2突变相关疾病。
4. **"JAK2 mutations and clinical practice: molecular mechanisms and diagnostic challenges"**
- **作者**: Vainchenker, W 等 (2008)
- **摘要**: 发表于《Haematologica》，解析JAK2信号通路异常机制，并评述基于抗体的磷酸化检测(如流式细胞术、Western blot)在评估JAK2激酶活性及药物疗效中的应用价值。
这些文献反映了JAK2抗体在突变检测、机制研究及临床诊断中的多维应用。
**Background of JAK2 Antibodies**
JAK2 (Janus kinase 2) is a tyrosine kinase critical for cytokine receptor signaling, particularly in hematopoiesis. It activates the STAT pathway, regulating cell proliferation, differentiation, and immune responses. Dysregulation of JAK2. especially the V617F mutation in exon 14. leads to constitutive kinase activity, driving myeloproliferative neoplasms (MPNs) like polycythemia vera and essential thrombocythemia.
JAK2 antibodies are tools used to detect JAK2 protein expression, phosphorylation status, or mutations. In diagnostics, they aid in identifying JAK2 mutations (via ELISA, PCR, or immunohistochemistry) to confirm MPNs. Research applications include studying JAK2-STAT signaling in cancer, inflammation, and autoimmune diseases. Therapeutic JAK2 inhibitors (e.g., ruxolitinib) target hyperactive JAK2. highlighting its clinical relevance.
Autoantibodies against JAK2 are rare but reported in autoimmune conditions, though their pathological role remains unclear. Overall, JAK2 antibodies bridge basic research and clinical practice, enabling mechanistic insights and precision medicine in JAK2-associated disorders.
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