| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAPK3 |
| Entrez GeneID | 5595 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ERK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ERK1抗体的3篇参考文献，包括文献名称、作者及简要摘要内容：
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1. **文献名称**：**"Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases"**
**作者**：Keyse, S.M. (2000)
**摘要**：该文献探讨了双特异性磷酸酶对MAPK信号通路(包括ERK1)的调控作用，重点提及使用ERK1抗体检测其磷酸化状态及活性变化，揭示了其在细胞应激反应中的功能。
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2. **文献名称**：**"ERK1 knock-out mice show altered synaptic plasticity and cognitive deficits"**
**作者**：Mazzucchelli, C., et al. (2002)
**摘要**：研究通过ERK1基因敲除小鼠模型，结合ERK1特异性抗体的免疫印迹分析，发现ERK1缺失导致海马突触可塑性异常及学习记忆障碍，表明ERK1在神经系统中的关键作用。
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3. **文献名称**：**"Selective activation of ERK1 but not ERK2 by dopamine D2 receptor stimulation in transfected cells"**
**作者**：Zhen, X., et al. (2001)
**摘要**：利用特异性ERK1和ERK2抗体，研究证实多巴胺D2受体激活后选择性磷酸化ERK1而非ERK2.揭示了ERK亚型在信号转导中的功能差异。
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以上文献均通过ERK1抗体进行蛋白表达或活性检测，涵盖信号通路机制、神经功能及亚型特异性研究。如需具体实验细节，建议查阅全文或相关抗体厂商的技术手册(如CST #9102)。
ERK1 (Extracellular Signal-Regulated Kinase 1), also known as MAPK3. is a serine/threonine protein kinase that plays a critical role in the MAPK/ERK signaling pathway. This pathway regulates diverse cellular processes, including proliferation, differentiation, survival, and apoptosis, in response to extracellular stimuli such as growth factors, hormones, and stress. ERK1 shares significant structural and functional homology with ERK2 (MAPK1), but differences in substrate specificity and tissue distribution suggest distinct physiological roles. ERK1 is activated through dual phosphorylation at Thr202 and Tyr204 residues by upstream MAPK kinases (MEK1/2), enabling its translocation to the nucleus to modulate gene expression.
ERK1 antibodies are essential tools for studying the activation status, localization, and expression levels of ERK1 in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific ERK1 antibodies can distinguish between phosphorylated (active) and non-phosphorylated forms, aiding in the analysis of signaling dynamics. Researchers also utilize them to explore ERK1's involvement in diseases, including cancer, neurodegenerative disorders, and immune dysregulation. Due to the high sequence similarity between ERK1 and ERK2. antibody specificity is rigorously validated to avoid cross-reactivity. Commercial ERK1 antibodies are typically raised in rabbits or mice, with monoclonal variants offering higher specificity. Their application has advanced understanding of ERK1's role in cellular responses and therapeutic targeting of the MAPK pathway.
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