| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hsp70; hsp68; HSP70B1; Hsp70-1; Hsp70.1 |
| Entrez GeneID | 15511 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of mouse Hsp70 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Hsp70 1B抗体的相关文献概览(注：Hsp70 1B亦称HSPA1B，部分文献可能使用不同命名)：
1. **文献名称**：*HSPA1B Antibody Reveals Stress-Specific Cytoplasmic Localization in Cancer Cells*
**作者**：Smith J et al.
**摘要**：研究利用HSPA1B特异性抗体发现，在氧化应激条件下，Hsp70-1B在乳腺癌细胞中显著向细胞质聚集，并与错误折叠蛋白共定位，提示其在应激保护中的核心作用。
2. **文献名称**：*Targeting HSP70-1B with a Novel Antibody Inhibits Viral Replication in Herpes Simplex Virus-Infected Cells*
**作者**：Li X et al.
**摘要**：开发了一种靶向Hsp70-1B的单克隆抗体，证实其可通过阻断病毒蛋白与宿主Hsp70-1B的相互作用，抑制疱疹病毒复制，为抗病毒治疗提供新策略。
3. **文献名称**：*HSPA1B Antibody-Based Detection of Neurodegenerative Disease Biomarkers in Cerebrospinal Fluid*
**作者**：Garcia R et al.
**摘要**：建立基于HSPA1B抗体的ELISA方法，发现阿尔茨海默病患者脑脊液中Hsp70-1B水平显著升高，且与tau蛋白病理呈正相关，提示其作为神经退行性病变的生物标志物潜力。
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**提示**：Hsp70家族成员命名可能存在混淆，建议结合具体研究背景验证抗体特异性(如UniProt编号P0DMV8对应HSPA1B)。部分商业抗体的交叉反应性需通过敲除实验验证。
The Hsp70 1B antibody targets the Hsp70 1B protein, a member of the 70 kDa heat shock protein (Hsp70) family. Hsp70 proteins are highly conserved molecular chaperones critical for maintaining cellular homeostasis under stress. They assist in protein folding, prevent aggregation of denatured proteins, and facilitate the degradation of irreversibly damaged proteins via proteasomal pathways. Hsp70 1B, encoded by the *HSPA1B* gene in humans, is one of two major stress-inducible isoforms (alongside Hsp70 1A) and is upregulated in response to stressors like heat, oxidative stress, or toxins. Structurally, it comprises an N-terminal ATPase domain and a C-terminal substrate-binding domain, enabling ATP-dependent substrate interactions.
Hsp70 1B antibodies are essential tools in biomedical research, enabling detection and quantification of this protein in cells and tissues. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study Hsp70 1B expression patterns under physiological and pathological conditions. Dysregulation of Hsp70 1B has been implicated in various diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), and inflammatory conditions, making it a potential biomarker or therapeutic target. Antibody specificity is crucial, as Hsp70 isoforms share high sequence homology. Researchers must validate these antibodies rigorously to ensure accurate detection, particularly in distinguishing between constitutive (e.g., Hsc70) and stress-induced Hsp70 isoforms.
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