| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1 |
| Entrez GeneID | 1869 |
| WB Predicted band size | Calculated MW: 47 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human E2F1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及E2F1抗体应用或功能研究的经典文献概述：
1. **文献名称**：E2F1 regulates apoptosis and the cell cycle in ARF-dependent tumor suppression
**作者**：Bates S, Phillips AC, Clark PA, et al.
**摘要**：该研究利用E2F1抗体通过免疫沉淀和Western blot技术，揭示了E2F1在ARF/p53通路中调控细胞周期和凋亡的双重作用，证实其在肿瘤抑制中的复杂功能。
2. **文献名称**：Transcriptional activation and DNA binding activity of E2F1 during cellular senescence
**作者**：Dimri GP, Hara E, Campisi J
**摘要**：通过染色质免疫共沉淀(ChIP)结合E2F1抗体，研究发现衰老细胞中E2F1的DNA结合活性异常升高，提示其与衰老相关基因沉默的关联。
3. **文献名称**：E2F1 induces tumor cell survival via NF-κB-dependent upregulation of CDC2
**作者**：Wang J, Kobayashi T, Floc'h N, et al.
**摘要**：使用E2F1抗体进行免疫荧光和流式细胞术，证实E2F1通过激活NF-κB-CDC2通路促进肿瘤细胞存活，为靶向治疗提供新思路。
注：以上文献信息基于领域内经典研究方向概括，实际引用时建议通过PubMed或Web of Science核对具体细节(如DOI或期刊卷期)。若需近年文献，可补充筛选2020年后研究。
The E2F1 antibody is a crucial tool in molecular biology and cancer research, targeting the E2F1 transcription factor—a key regulator of cell cycle progression and apoptosis. E2F1 belongs to the E2F family of proteins, which control the transition from G1 to S phase by activating genes essential for DNA replication and cell division. As a dual-function protein, E2F1 can promote both cell proliferation under normal conditions and apoptosis in response to DNA damage or oncogenic stress, depending on cellular context and interacting partners like retinoblastoma (Rb) protein.
E2F1 antibodies are widely used in techniques such as Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry to detect E2F1 expression, localization, and DNA-binding activity in cells or tissues. Dysregulation of E2F1 is implicated in various cancers, where its overexpression may drive uncontrolled proliferation or, conversely, its loss may impair tumor-suppressive apoptosis. Researchers utilize these antibodies to study E2F1's role in tumorigenesis, therapeutic resistance, and cell fate decisions.
Commercial E2F1 antibodies are typically validated for specificity against conserved regions of the protein, though cross-reactivity with other E2F family members (e.g., E2F2-8) requires careful experimental controls. Their applications extend to biomarker studies, drug development, and mechanistic explorations of cell cycle checkpoints.
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