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产品描述：马胰岛素样生长因子1(IGF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF1含量。IGF1即胰岛素样生长因子 1，在人体生长发育、细胞增殖分化等生理过程中发挥重要作用。其异常表达与癌症、糖尿病等疾病密切相关。研究机制多聚焦于它与受体结合激活下游信号通路，以探索通过干预该靶点治疗相关疾病的策略。试剂盒检测范围为12.5 ng/mL-800 ng/mL，适用于马属动物相关的基础研究，如运动生理学分析、生长调控机制探索、代谢性疾病模型构建等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit; Fragment ELISA Kit
缩写：IGF1
Uniprot No.：P51458
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-104 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 80-97 | |||||
回收率：
| The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 87-101 | ||||
| EDTA plasma (n=4) | 87 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8001.9611.9441.9531.8204001.4881.6051.5471.4142001.1781.2131.1961.0631000.9120.9020.9070.774500.6690.6570.6630.530250.4050.3960.4010.26812.50.2600.2490.2550.12200.1370.1290.133 | ||||||
货期：3-5 working days
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