| WB | 1/500-1/1000 | Human,Mouse,Rat,Hamster |
| IF | 咨询技术 | Human,Mouse,Rat,Hamster |
| IHC | 1/50-1/100 | Human,Mouse,Rat,Hamster |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Hamster |
| FCM | 咨询技术 | Human,Mouse,Rat,Hamster |
| Elisa | 咨询技术 | Human,Mouse,Rat,Hamster |
| Aliases | VIM; Vimentin |
| Entrez GeneID | 7431 |
| WB Predicted band size | Calculated MW: 54 kDa; Observed MW: 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Hamster |
| Immunogen | A synthetic peptide of human Vimentin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于Vimentin抗体的参考文献及其简要摘要：
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1. **文献名称**: *"Vimentin in cancer and its potential as a molecular target for cancer therapy"*
**作者**: Satelli A, Li S.
**摘要**: 本文综述了Vimentin在肿瘤上皮-间质转化(EMT)和转移中的作用，强调其作为转移性癌症生物标志物的价值。研究探讨了Vimentin抗体在检测肿瘤细胞迁移和侵袭中的应用，及其在靶向治疗中的潜力。
2. **文献名称**: *"The role of intermediate filament proteins in the regulation of cell motility during epithelial-mesenchymal transition"*
**作者**: Kalluri R.
**摘要**: 研究分析了中间纤维蛋白(如Vimentin)在EMT过程中的功能，利用特异性抗体证实Vimentin在维持细胞形态和迁移中的关键作用，并探讨其在肿瘤微环境中的动态表达模式。
3. **文献名称**: *"Monoclonal antibodies specific for vimentin: characterization and application in pathology"*
**作者**: Langhofer M, et al.
**摘要**: 该研究比较了多种商品化Vimentin抗体在免疫组化(IHC)和免疫印迹(WB)中的特异性与灵敏度，验证了其在识别正常组织与肿瘤中Vimentin表达的可靠性，为病理诊断提供方法学参考。
4. **文献名称**: *"Vimentin phosphorylation as a target for cell cycle-dependent regulation of intermediate filaments"*
**作者**: Konieczny P, et al.
**摘要**: 通过磷酸化特异性Vimentin抗体，研究揭示了细胞周期中Vimentin的翻译后修饰机制，及其在细胞分裂和迁移中的功能调控，强调了抗体在解析细胞动力学中的工具价值。
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以上文献涵盖了Vimentin抗体的应用场景(如肿瘤研究、细胞动力学)、实验方法学验证及其在分子机制研究中的作用。如需具体期刊或年份，可进一步补充说明。
Vimentin, a type III intermediate filament protein, is a key structural component of the cytoskeleton in mesenchymal cells. It plays essential roles in maintaining cell integrity, organelle positioning, and facilitating cell migration, adhesion, and signaling. Vimentin is broadly expressed during embryonic development but becomes restricted to cells of mesenchymal origin (e.g., fibroblasts, endothelial cells, and immune cells) in adults. Its expression is dynamically regulated during epithelial-mesenchymal transition (EMT), a process critical in wound healing, metastasis, and fibrosis.
Vimentin antibodies are widely used tools in research and diagnostics to detect vimentin in tissues or cultured cells. These antibodies, often generated in hosts like mice or rabbits, target specific epitopes of the protein and are validated for techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help identify mesenchymal cell populations, assess EMT in cancer progression, and study diseases involving cytoskeletal dysregulation, including fibrosis, neurodegenerative disorders, and autoimmune conditions. In clinical pathology, vimentin antibodies aid in diagnosing sarcomas and other mesenchymal tumors. Researchers also utilize them to explore vimentin’s non-canonical roles, such as its interaction with pathogens or involvement in inflammatory responses. Variants like phospho-specific antibodies further enable studies on post-translational modifications regulating vimentin function. Overall, vimentin antibodies are indispensable for understanding cellular dynamics and disease mechanisms linked to the cytoskeleton.
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