| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANXA6 |
| Uniprot No | P08133 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-245aa |
| 氨基酸序列 | AKPAQGAKYRGSIHDFPGFDPNQDAEALYTAMKGFGSDKEAILDIITSRSNRQRQEVCQSYKSLYGKDLIADLKYELTGKFERLIVGLMRPPAYCDAKEIKDAISGIGTDEKCLIEILASRTNEQMHQLVAAYKDAYERDLEADIIGDTSGHFQKMLVVLLQGTREEDDVVSEDLVQQDVQDLYEAGELKWGTDEAQFIYILGNRSKQHLRLVFDEYLKTTGKPIEASIRGELSGDFEKLMLAV |
| 预测分子量 | 54.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANXA6重组蛋白的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*Recombinant ANXA6 modulates membrane repair in cancer cells*
**作者**：Lee, S. et al.
**摘要**：研究利用重组ANXA6蛋白，发现其通过促进细胞膜修复机制抑制乳腺癌细胞迁移，揭示了ANXA6在癌症转移中的潜在治疗作用。
2. **文献名称**：*Expression and purification of human ANXA6 in E. coli for lipid-binding assays*
**作者**：Zhang, Y. & Kumar, R.
**摘要**：报道了一种高效的大肠杆菌表达系统制备重组ANXA6蛋白的方法，并验证其与磷脂酰丝氨酸的特异性结合能力，为后续功能研究提供工具。
3. **文献名称**：*ANXA6重组蛋白通过调控TGF-β信号通路抑制肝纤维化*
**作者**：Wang, J. et al.
**摘要**：在小鼠模型中，外源性重组ANXA6蛋白通过抑制TGF-β/Smad3通路减轻肝星状细胞活化，提示其作为抗纤维化靶点的潜力。
注：以上文献为模拟内容，实际研究需通过PubMed、Web of Science等平台检索。
**Background of ANXA6 Recombinant Protein**
ANXA6. a member of the annexin protein family, is a calcium-dependent phospholipid-binding protein implicated in diverse cellular processes, including membrane organization, signal transduction, and intracellular trafficking. This 68-70 kDa protein is structurally characterized by eight conserved annexin repeats, divided into two domains connected by a flexible linker, which enable interactions with membranes, cytoskeletal components, and signaling molecules. ANXA6 is ubiquitously expressed but shows elevated levels in tissues like the liver, brain, and skeletal muscle, where it regulates cholesterol homeostasis, endocytic pathways, and cell adhesion.
The recombinant ANXA6 protein is generated using heterologous expression systems (e.g., *E. coli*, mammalian, or insect cells*) to produce a purified, functional form for research applications. Recombinant technology allows precise control over post-translational modifications, enhancing reproducibility in studies. ANXA6's role in disease contexts, such as cancer, cardiovascular disorders, and diabetes, has driven interest in its recombinant form. For instance, it acts as a tumor suppressor in certain cancers by modulating Ras/MAPK signaling, while in others, it promotes metastasis via interactions with extracellular matrix components.
Research utilizing ANXA6 recombinant protein has clarified its molecular mechanisms, including its ability to scaffold membrane microdomains, influence calcium signaling, and mediate membrane repair. Its involvement in viral entry (e.g., hepatitis C virus) and lysosomal storage disorders further underscores its biomedical relevance. Ongoing studies aim to explore its therapeutic potential, leveraging recombinant ANXA6 to dissect pathways or develop targeted interventions. Overall, ANXA6 recombinant protein serves as a vital tool for unraveling the multifunctional roles of this annexin in health and disease.
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