| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB. |
| Entrez GeneID | 2959 |
| WB Predicted band size | Calculated MW: 35 kDa; Observed MW: 35 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human TFIIB |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Transcription Initiation Factor IIB(TFIIB)抗体的代表性文献：
1. **"Structure of transcription factor TFIIB in a DNA polymerase II preinitiation complex"**
- **作者**: Hahn, S., et al. (1996)
- **摘要**: 该研究通过免疫沉淀和结构分析，验证了TFIIB抗体在识别转录起始复合物中的特异性，揭示了TFIIB与RNA聚合酶II及启动子DNA的相互作用模式。
2. **"Multiple functional domains of human transcription factor IIB: Unique activator-targeting determinants in an evolutionarily conserved core"**
- **作者**: Ha, I., et al. (1993)
- **摘要**: 利用特异性抗体鉴定TFIIB的功能域，证明其N端和C端区域在转录激活中的关键作用，并验证抗体在体外转录实验中的阻断效果。
3. **"Crystal structure of a TFIIB-TBP-TATA-element ternary complex"**
- **作者**: Nikolov, D.B., et al. (1995)
- **摘要**: 通过X射线晶体学解析TFIIB与TBP及DNA的复合物结构，研究中使用的TFIIB抗体被用于验证蛋白复合物的组装与功能。
4. **"The role of human transcription factor IIB in promoter escape and regulation during early elongation"**
- **作者**: Schultz, M.C., et al. (2000)
- **摘要**: 利用TFIIB抗体进行染色质免疫沉淀(ChIP)，证明TFIIB在转录起始向延伸阶段的动态解离过程及其调控机制。
以上文献均涉及TFIIB抗体的应用，涵盖结构分析、功能域鉴定及动态调控研究。
Transcription Initiation Factor IIB (TFIIB) is a critical component of the RNA polymerase II (Pol II) transcription machinery, playing a central role in the assembly of the pre-initiation complex (PIC) at gene promoters. As a general transcription factor, TFIIB bridges RNA Pol II and promoter-bound TFIID/TATA-binding protein (TBP), facilitating promoter recognition and transcription initiation. Structurally, it contains an N-terminal zinc ribbon domain involved in Pol II interaction and a C-terminal core domain for DNA binding and TFIID/TBP association.
Antibodies targeting TFIIB are essential tools for studying transcriptional regulation mechanisms. They are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to investigate TFIIB's expression, localization, and interactions within cellular complexes. These antibodies help elucidate TFIIB’s dynamic role in transcription cycles, including its recruitment to promoters, stabilization of PIC, and potential involvement in transcription reinitiation.
Given TFIIB’s conserved nature across eukaryotes, such antibodies often exhibit cross-reactivity in model organisms, supporting comparative studies in gene expression regulation. Researchers also utilize TFIIB antibodies to explore dysregulation in diseases linked to transcriptional defects, including cancers and neurological disorders. Specific epitope-targeted antibodies (e.g., recognizing phosphorylated or modified TFIIB forms) further enable studies on post-translational regulation of transcription initiation. Validation typically includes knockout/knockdown controls to ensure specificity in experimental systems.
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