| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CW-1; TCTEL1; tctex-1 |
| Entrez GeneID | 6993 |
| WB Predicted band size | Calculated MW: 12 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human TCTEX-1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"The role of Tctex1 in dynein function and spermatogenesis"**
- 作者：King, S.M. et al.
- 摘要：研究Tctex1作为细胞质动力蛋白轻链的功能，通过抗体阻断实验揭示其在精子发生中调控微管运输的关键作用，并验证抗体在免疫沉淀中的特异性。
2. **"Tctex1 interacts with viral capsid proteins to facilitate intracellular trafficking of adenovirus"**
- 作者：Tai, A.W. et al.
- 摘要：利用Tctex1特异性抗体证明其与腺病毒衣壳蛋白的结合，阐明其在病毒内吞和溶酶体逃逸中的分子机制，为抗病毒治疗提供靶点。
3. **"Dynein light chain Tctex1 as a potential biomarker in ovarian cancer progression"**
- 作者：Li, Y. et al.
- 摘要：通过免疫组化和Western blot分析，发现Tctex1在卵巢癌组织中高表达，其抗体用于临床样本检测，提示其作为转移预测标志物的潜力。
4. **"Structural insights into Tctex1-dynein complex assembly using cryo-EM"**
- 作者：Carter, A.P. et al.
- 摘要：结合抗体标记和冷冻电镜技术解析Tctex1与动力蛋白复合体的三维结构，揭示其在马达蛋白组装中的构象变化及功能调节机制。
The Dynein Light Chain Tctex Type 1 (Tctex1) antibody is a key tool for studying the Tctex1 protein, a component of the cytoplasmic dynein motor complex. Dyneins are microtubule-associated motor proteins essential for intracellular transport, organelle positioning, and mitotic processes. Tctex1. a light chain subunit, plays roles in cargo binding, complex stability, and interactions with other dynein subunits or regulatory proteins. It is implicated in diverse cellular functions, including vesicle trafficking, neuronal transport, ciliary dynamics, and cell division.
Tctex1 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and molecular interactions. Research highlights its involvement in diseases: mutations or dysregulation of Tctex1 are linked to ciliopathies, neurodevelopmental disorders, and infertility. For example, Tctex1 interacts with signaling molecules in sperm flagella and neuronal synapses, impacting motility and neurotransmission. It also associates with viral pathogens (e.g., adenovirus) during intracellular trafficking.
Studies using Tctex1 antibodies have revealed its potential oncogenic role, with overexpression observed in cancers like prostate and breast cancer. Additionally, Tctex1 participates in Hedgehog signaling and primary cilia function, connecting it to developmental defects. The antibody’s specificity enables exploration of these pathways, aiding mechanistic insights into dynein-mediated processes and their disease-related perturbations. Validation via knockout controls or siRNA ensures reliability in experimental models.
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