| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RCC1; RCC1-I; SNHG3-RCC1; CHC1 |
| Entrez GeneID | 1104 |
| WB Predicted band size | Calculated MW: 45 kDa; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human RCC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RCC1抗体的3篇参考文献，按研究方向和抗体应用场景分类整理：
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### 1. **细胞周期调控研究**
**文献名称**：*Human RCC1 is required for proper mitotic spindle assembly and chromosome segregation*
**作者**：Ohtsubo, M., et al.
**摘要**：
研究通过免疫荧光和Western blot技术，利用RCC1特异性抗体，揭示RCC1在纺锤体形成和染色体分离中的关键作用。抗体实验显示RCC1缺陷导致细胞周期停滞在中期，证实其调控染色体动态的功能。
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### 2. **癌症相关表达分析**
**文献名称**：*Overexpression of RCC1 correlates with poor prognosis and promotes tumor invasion in hepatocellular carcinoma*
**作者**：Li, Y., et al.
**摘要**：
该研究使用RCC1抗体进行免疫组化分析肝癌组织，发现RCC1高表达与患者生存率低及肿瘤转移相关。实验进一步通过敲低RCC1抑制癌细胞侵袭，提示其作为肝癌治疗靶点的潜力。
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### 3. **核运输机制探索**
**文献名称**：*RCC1-dependent nuclear transport of Ran GTPase regulates cell cycle progression*
**作者**：Bischoff, F.R., et al.
**摘要**：
利用RCC1抗体进行免疫沉淀和亚细胞定位分析，发现RCC1通过调控Ran蛋白的核质穿梭影响细胞周期进程。抗体阻断实验表明RCC1-Ran互作是核运输功能的核心机制。
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### 可选补充(技术验证方向)
**文献名称**：*Characterization of a monoclonal antibody specific to RCC1 for diagnostic applications*
**作者**：Suzuki, T., et al.
**摘要**：
研究报道了一种高特异性RCC1单克隆抗体的开发，通过ELISA、流式细胞术验证其灵敏度和特异性，为癌症诊断和基础研究提供可靠工具。
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**应用场景提示**：
- **抗体选择**：需关注文献中抗体的宿主来源(如兔/鼠单抗)及适用实验(WB/IF/IHC)。
- **疾病关联**：肝癌、乳腺癌等研究中RCC1的表达分析可参考Li等人的方法。
- **机制研究**：涉及细胞周期或核运输的课题可优先引用Ohtsubo和Bischoff的经典研究。
建议通过PubMed或Web of Science用关键词“RCC1 antibody” + “Western blot/IF/IHC”进一步筛选近期高引论文。
The Regulator of Chromosome Condensation 1 (RCC1) is a critical protein involved in cell cycle regulation, primarily functioning as a guanine nucleotide exchange factor (GEF) for the small GTPase Ran. RCC1 binds to chromatin via interaction with histones H2A/H2B and plays a pivotal role in maintaining nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation during mitosis. It is characterized by a seven-bladed β-propeller structure, enabling dynamic association with chromatin to regulate RanGTP gradients essential for cellular processes.
RCC1 antibodies are widely used tools in molecular and cell biology research to investigate RCC1 expression, localization, and function. These antibodies, often generated in rabbits or mice through immunization with recombinant RCC1 protein fragments, enable detection of endogenous RCC1 via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies employing RCC1 antibodies have elucidated its role in cancer biology, particularly in malignancies linked to chromosomal instability, such as renal cell carcinoma and leukemia. Dysregulation of RCC1-Ran signaling is associated with aberrant cell proliferation and compromised genome integrity. Additionally, RCC1 antibodies aid in exploring cellular responses to DNA damage and chemotherapeutic agents targeting mitotic machinery. Validation typically includes knockout cell lines or siRNA-mediated depletion controls to ensure specificity.
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