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产品描述：人肠型碱性磷酸酶(ALPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ALPI含量。ALPI即碱性磷酸酶肠同工酶。目前研究发现它与多种生理病理过程有关，如参与肠道内物质的代谢与吸收。其研究机制主要聚焦于它在肠道微环境中的作用，以及与肠道疾病、代谢性疾病发生发展的关联，未来有望成为相关疾病诊治新靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，为肠道功能研究、疾病模型建立、营养代谢机制探索等基础科研领域提供可靠工具，尤其适用于评估肠道健康状态、炎症性肠病机制研究或药物干预效果分析等实验场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Alkaline phosphatase intestinal ELISA Kit; Alkaline phosphomonoesterase ELISA Kit; Alpi ELISA Kit; Glycerophosphatase ELISA Kit; IAP ELISA Kit; Intestinal alkaline phosphatase ELISA Kit; Intestinal-type alkaline phosphatase ELISA Kit; Kasahara isozyme ELISA Kit; OTTHUMP00000164357 ELISA Kit; PPBI_HUMAN ELISA Kit
缩写：ALP
Uniprot No.：P09923
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human ALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-96 | ||||
| EDTA plasma (n=4) | 95 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4682.4542.4612.3411001.8401.7761.8081.688501.2541.1641.2091.089250.7830.7390.7610.64112.50.4840.5010.4930.3736.250.3190.3240.3220.2023.120.2020.2110.2070.08700.1180.1220.120 | ||||||
货期：3-5 working days
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